IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v52y2007i9id1997-vetmed.html
   My bibliography  Save this article

Isolation of conditionally pathogenic mycobacteria from the environment of one pig farm and the effectiveness of preventive measures between 1997 and 2003

Author

Listed:
  • I. Pavlik

    (Veterinary Research Institute, Brno, Czech Republic)

  • L. Matlova

    (Veterinary Research Institute, Brno, Czech Republic)

  • M. Gilar

    (State Veterinary District Administration, Pelhrimov, Czech Republic)

  • J. Bartl

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Parmova

    (State Veterinary Institute, Prague, Czech Republic)

  • F. Lysak

    (Field Veterinarian, Pelhrimov, Czech Republic)

  • M. Alexa

    (Veterinary Research Institute, Brno, Czech Republic)

  • L. Dvorska-Bartosova

    (Veterinary Research Institute, Brno, Czech Republic)

  • V. Svec

    (State Veterinary District Administration, Pelhrimov, Czech Republic)

  • V. Vrbas

    (Veterinary Research Institute, Brno, Czech Republic)

  • A. Horvathova

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

Between 1997 and 2003, in one herd of breeding pigs with 90 sows and two boars, positive and dubious responses to avian tuberculin were detected in 4 and 72 pigs, respectively. Pigs were examined using the agglutination test for the presence of serum antibodies against corpuscular antigens prepared from various Mycobacterium avium complex (MAC) members: M. a. avium (MAA) of serotype 2, M. a. hominissuis (MAH) of serotype 8 and M. intracellulare (MI) of serotype 19. Positive skin responses were found in animals with antibodies against MAH (18; 23.7%), MAA (3; 4.0%) and MI (9; 11.8%) antigens. By serological examination of 17 sows with repeated dubious responses for tuberculin skin testing with avian tuberculin, no antibodies against MAA were detected; MAH antibodies and MI antibodies were found in eight and two animals, respectively. By post mortem examination of lymph nodes (ln) and organ samples from all 76 animals with responses to avian tuberculin, no tuberculous/tuberculoid lesions were detected. By culture examination of ln and organs from 13 animals, conditionally pathogenic mycobacteria (CPM) were isolated from only one animal (breeding boar): from mesenteric, pulmonary, hepatic ln and from spleen tissue samples. These isolates were identified as MAH and CPM by the PCR method and biochemically. By investigation of the external environment (205 samples), 33 (16.3%) CPM isolates were obtained: 13 MAH, eight M. fortuitum, one M. nonchromogenicum, one M. abscessus, one M. scrofulaceum and nine unidentified isolates, which were non-MAC according to the PCR examination. Non-specific responses obtained in the intravital tests (skin and serological tests) caused by CPM present in the environment substantially complicated diagnosis of avian tuberculosis. Based on these findings, animal hygiene measures have been adopted since 2002; resulted in a decrease of environmental contamination with CPM and a reduction in the number of animals giving positive responses to avian tuberculin.

Suggested Citation

  • I. Pavlik & L. Matlova & M. Gilar & J. Bartl & I. Parmova & F. Lysak & M. Alexa & L. Dvorska-Bartosova & V. Svec & V. Vrbas & A. Horvathova, 2007. "Isolation of conditionally pathogenic mycobacteria from the environment of one pig farm and the effectiveness of preventive measures between 1997 and 2003," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 52(9), pages 392-404.
    • Handle: RePEc:caa:jnlvet:v:52:y:2007:i:9:id:1997-vetmed
    DOI: 10.17221/1997-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1997-VETMED.html
    Download Restriction: free of charge
    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1997-VETMED.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/1997-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. I. Pavlik & W. Yayo Ayele & I. Parmova & I. Melicharek & M. Hanzlikova & B. Körmendy & G. Nagy & Z. Cvetnic & M. Ocepek & N. Fejzic & M. Lipiec, 2002. "Incidence of bovine tuberculosis in cattle in seven Central European countries during the years 1990-1999," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 47(2-3), pages 45-51.
    2. A. Kozak & V. Vecerek & P. Chloupek & B. Tremlova & M. Malena, 2003. "Veterinary meat inspection of pig carcasses in the Czech Republic during the period of 1995-2002," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 48(8), pages 207-214.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. A. Kozak & J. Holejsovsky & P. Belobradek & L. Ostadalova & P. Chloupek, 2004. "Emergency slaughter of pigs due to immobility," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(10), pages 359-364.
    2. L. Matlova & L. Dvorska & J. Bartl & M. Bartos & W.Y. Ayele & M. Alexa & I. Pavlik, 2003. "Mycobacteria isolated from the environment of pig farms in the Czech Republic during the years 1996 to 2002," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 48(12), pages 343-357.
    3. V. Vecerek & M. Malena & M. Malena & E. Voslarova & P. Chloupek, 2006. "The impact of the transport distance and season on losses of fattened pigs during transport to the slaughterhouse in the Czech Republic in the period from 1997 to 2004," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(1), pages 21-28.
    4. I. Pavlik & L. Matlova & L. Dvorska & J.E. Shitaye & I. Parmova, 2005. "Mycobacterial infections in cattle and pigs caused by Mycobacterium aviumcomplex members and atypical mycobacteria in theCzech Republicduring 2000-2004," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(7), pages 281-290.
    5. I. Pavlik & I. Trcka & I. Parmova & J. Svobodova & I. Melicharek & G. Nagy & Z. Cvetnic & M. Ocepek & M. Pate & M. Lipiec, 2005. "Detection of bovine and human tuberculosis in cattle and other animals in six Central European countries during the years 2000-2004," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(7), pages 291-299.
    6. A. Kozak & M. Malena & J. Holejsovsky & B. Bartosek, 2004. "Emergency slaughters in pigs in the Czech Republic during the period of 1997-2002," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(10), pages 365-369.
    7. L. Pavlata & A. Podhorsky & A. Pechova & P. Chomat, 2005. "Differences in the occurrence of selenium, copper and zinc deficiencies in dairy cows, calves, heifers and bulls," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(9), pages 390-400.

    More about this item

    Keywords

    IS901; IS1245; dnaJ; zoonosis; avian mycobacteriosis; food safety; ecology;
    All these keywords.

    JEL classification:

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:52:y:2007:i:9:id:1997-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.