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Commercial phenotypic tests (API 20E) in diagnosis of fish bacteria: a review

Author

Listed:
  • N. Topic Popovic

    (Rudjer Boskovic Institute, Zagreb, Croatia)

  • R. Coz-Rakovac

    (Rudjer Boskovic Institute, Zagreb, Croatia)

  • I. Strunjak-Perovic

    (Rudjer Boskovic Institute, Zagreb, Croatia)

Abstract

The available data concerning rapid identification of fish bacteria via commercial phenotypic tests demonstrate that there is no agreement regarding the choice of the tests. However, API 20E, an identification system for Enterobacteriaceae and other non-fastidious Gram-negative rods developed for clinical specimens, seems to be increasingly used for the identification of fish pathogens. In this review, adaptation of API 20E for fish bacterial isolates and its distinctiveness for fish bacteria was assessed. Some strains are wrongly identified because they are not included in the database of API 20E system. API 20E reactions should be compared with the diagnostic schemes based on reactions in conventional phenotypic tests. Due to their significance for fish health and impact on the aquaculture, and because of the need for their rapid identification, some important fish bacteria should be included in the API 20E system, such as Yersinia ruckeri, Edwardsiella ictaluri, Vibrio anguillarum.

Suggested Citation

  • N. Topic Popovic & R. Coz-Rakovac & I. Strunjak-Perovic, 2007. "Commercial phenotypic tests (API 20E) in diagnosis of fish bacteria: a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 52(2), pages 49-53.
  • Handle: RePEc:caa:jnlvet:v:52:y:2007:i:2:id:2058-vetmed
    DOI: 10.17221/2058-VETMED
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    References listed on IDEAS

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    1. R. R Čož-Rakovac & I. Strunjak-Perović & N. Topić Popović & M. Hacmanjek & B. Šimpraga & E. Teskeredžić, 2002. "Health status of wild and cultured sea bass in the northern Adriatic Sea," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 47(8), pages 222-226.
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    Cited by:

    1. I. Sedláček & E. Staňková & P. Švec, 2016. "Composition of cultivable enteric bacteria from the intestine of Antarctic fish (family Nototheniidae)," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 61(3), pages 127-132.

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      Keywords

      API 20E; bacteria; diagnostics; fish;
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