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Rapid differentiation of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis by amplification of insertion element IS901

Author

Listed:
  • P. Svastova

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Pavlik

    (Veterinary Research Institute, Brno, Czech Republic)

  • M. Bartos

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

The aim of this study was to examine the specificity of primers designed to detect the insertion element IS901 commonly used in differentiation of Mycobacterium avium complex strains. This study shows that one of these primers non-specifically anneals to a sequence inside insertion element IS900, specific IS of M. avium subsp. paratuberculosis and to another sequence flanking this element. The resulting non-specific amplicon can be a product of amplification from some M. avium subsp. paratuberculosis strains and can simulate the presence of insertion element IS901 in these strains. However size difference between specific and non-specific amplicons allows such false-positive results to be distinguished. In addition the single PCR allows a rapid and simple differentiation between IS901+ M. avium subsp. avium and M. avium subsp. paratuberculosis strains.

Suggested Citation

  • P. Svastova & I. Pavlik & M. Bartos, 2002. "Rapid differentiation of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis by amplification of insertion element IS901," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 47(5), pages 117-121.
    • Handle: RePEc:caa:jnlvet:v:47:y:2002:i:5:id:5814-vetmed
    DOI: 10.17221/5814-VETMED
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    Cited by:

    1. T. Amemori & L. Matlova & O. A Fischer & W. Y Ayele & M. Machackova & E. Gopfert & I. Pavlik, 2004. "Distribution of Mycobacterium avium subsp. paratuberculosis in the gastrointestinal tract of shedding cows and its application to laparoscopic biopsy," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(7), pages 225-236.
    2. J.E. Shitaye & R. Halouzka & J. Svobodova & V. Grymova & M. Grym & M. Skoric & P. Fictum & V. Beran & M. Slany & I. Pavlik, 2010. "First isolation of Mycobacterium genavense in a blue headed parrot (Pionus menstruus) imported from Surinam (South America) to the Czech Republic: a case report," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 55(7), pages 339-347.
    3. J. Hosek & P. Svastova & M. Moravkova & I. Pavlik & M. Bartos, 2006. "Methods of mycobacterial DNA isolation from different biological material: a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(5), pages 180-192.
    4. K. Hruska, 2004. "Research on paratuberculosis: Analysis of publications 1994-2004," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(8), pages 271-282.
    5. I. Pavlik & P. Jahn & L. Dvorska & M. Bartos & L. Novotny & R. Halouzka, 2004. "Mycobacterial infections in horses: a review of the literature," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(11), pages 427-440.

    More about this item

    Keywords

    IS901; IS900; PCR; Johne's diseases; avian tuberculosis;
    All these keywords.

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