IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v47y2002i1id5797-vetmed.html
   My bibliography  Save this article

Respiratory burst in neutrophilic granulocytes of carps (Cyprinus carpio): cytometric studies

Author

Listed:
  • M. Stosik

    (University of Zielona Góra, Zielona Góra, Poland)

  • W. Deptula

    (University of Zielona Góra, Zielona Góra, Poland)

  • K. Wiktorowicz

    (University of Zielona Góra, Zielona Góra, Poland)

  • M. Trávníček

    (University of Zielona Góra, Zielona Góra, Poland)

  • K. Baldy-Chudzik

    (University of Zielona Góra, Zielona Góra, Poland)

Abstract

Present studies aimed at flow cytometric analysis of respiratory burst in neutrophilic granulocytes in carps, at various stages of their ontogeny. Cytometric evaluation of the repiratory burst in PMN cells of carps demonstrated augmented values of the mean fluorescence channel of PMA-stimulated PMN cells, as compared to the value of the fluorescence channel noted for PMN cells which were not activated or did not respond to activation in response to the applied stimulator. Intensity of the fluorescence, compared to the mean fluorescence channel of the cells which were not stimulated by PMA, was most pronounced in 11- to 21-month old carps, average in the youngest carps aging 3 to 9 months, and the lowest in the eldest, 23- to 29-month old carps. In cases of analysis of the mean fluorescence channel in the selected fraction of PMA-stimulated PMN cells, as compared to the mean fluorescence channel of granulocytes which did not respond by the stimulation reaction, the most intense fluorescence was noted to develop in the eldest, i.e. 23- to 29-month old fish. In the remaining carps, on the other hand, the difference in the mean fluorescence channel was significantly lower. In parallel, the highest fraction of PMA-stimulated granulocytes was found in 11- to 21-month old carps, a lower fraction in the youngest carps aging 3 to 9 months and the lowest fraction of such granulocytes in the eldest carps, 23 to 29 months of age.

Suggested Citation

  • M. Stosik & W. Deptula & K. Wiktorowicz & M. Trávníček & K. Baldy-Chudzik, 2002. "Respiratory burst in neutrophilic granulocytes of carps (Cyprinus carpio): cytometric studies," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 47(1), pages 17-20.
    • Handle: RePEc:caa:jnlvet:v:47:y:2002:i:1:id:5797-vetmed
    DOI: 10.17221/5797-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5797-VETMED.html
    Download Restriction: free of charge
    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5797-VETMED.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/5797-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:47:y:2002:i:1:id:5797-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.