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The use of the computer technology for the evaluation of the strict morphological sperm analysis

Author

Listed:
  • Z. Věžník

    (Veterinary Research Institute, Brno, Czech Republic)

  • O. Matoušková

    (Veterinary Research Institute, Brno, Czech Republic)

  • D. Švecová

    (Veterinary Research Institute, Brno, Czech Republic)

  • A. Zajícová

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

A programme for evaluating the strict morphological analysis of sperm was developed. The programme was verified by conducting 552 morphological analyses of bull, stallion, boar and human ejaculates. The method was evaluated by comparing the results obtained by routinely used morphological examinations with the results received by the automatic method SASMO. The following advantages of the programme were demonstrated: it is possible to obtain information on the proportion of normal and pathological spermatozoa, to determine the frequency of the respective characteristics analysed, to express detected alterations per pathological spermatozoon (teratosperm index), to express frequency of respective alterations per total number of spermatozoa examined, to display alterations according to their frequency and differentiate between developmental and acquired alterations. It was proved that using the detailed morphological evaluation of sperm by the survival test, it is possible to get statistically significant information on the prediction of sperm survival. The programme represents an important aid for making the morphological evaluation of sperm quality more objective both in veterinary and human medicine and in all insemination stations and assisted reproduction centres.

Suggested Citation

  • Z. Věžník & O. Matoušková & D. Švecová & A. Zajícová, 2001. "The use of the computer technology for the evaluation of the strict morphological sperm analysis," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 46(2), pages 35-40.
    • Handle: RePEc:caa:jnlvet:v:46:y:2001:i:2:id:7849-vetmed
    DOI: 10.17221/7849-VETMED
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    Cited by:

    1. Z. Veznik & D. Svecova & A. Zajicova & Z. Reckova & J. Rubes, 2007. "The interrelationship between quality parameters of sperm before and after separation by gradient centrifugation," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 52(10), pages 423-429.
    2. P. Prinosilova & Z. Veznik & A. Zajicova & D. Svecova, 2005. "Using the Sperm Quality Analyzer (SQA IIc) to evaluate dog ejaculates," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(5), pages 195-204.

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