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Effects of homobrassinolide in barley callus culture

Author

Listed:
  • A. Temel

    (Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Vezneciler, Istanbul, Turkey)

  • N. Gozukirmizi

    (Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Vezneciler, Istanbul, Turkey)

Abstract

Thirty-day-old barley (Hordeum vulgare L. cv. Zafer-160) calli were treated with 0, 0.5, 1 µmol/L 22(S),23(S)-Homobrassinolide (HBL) for four weeks. Transfer of calli to hormone-free or HBL-supplemented medium caused an increase in total soluble protein and DNA contents and a decrease in superoxide dismutase (SOD; E.C. 1.15.1.1) activity. 0.5 µmol/L HBL caused the highest protein and DNA levels and the minimum decrease in fresh weight; while the lowest superoxide dismutase activity was recorded in 1 µmol/L HBL-treated group. Calli developed nodular structures and green cell clusters after transfer onto hormone-free or HBL-supplemented medium. Methylation-sensitive restriction fingerprinting (MSRF) analysis detected a few changes in methylation between control and 0.5 µmol/L groups. Combination of data led us to the conclusion that HBL favoured regeneration and shoot development and may ameliorate tissue culture stress in a mechanism, which is independent of cytosine methylation.

Suggested Citation

  • A. Temel & N. Gozukirmizi, 2012. "Effects of homobrassinolide in barley callus culture," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 58(10), pages 441-445.
    • Handle: RePEc:caa:jnlpse:v:58:y:2012:i:10:id:305-2012-pse
    DOI: 10.17221/305/2012-PSE
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    References listed on IDEAS

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    1. S. Anuradha & S.S.R. Rao, 2007. "The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 53(11), pages 465-472.
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