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Expression of OsNHX1 gene in maize confers salt tolerance and promotes plant growth in the field

Author

Listed:
  • M. Chen

    (State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China
    State Key Laboratory for Microbial Technology, College of Biological Sciences, Shandong University, Jinan, China)

  • Q.-J. Chen

    (State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China)

  • X.-G. Niu

    (College of Land and Environment, Shenyang Agricultural University, Shenyang, China)

  • R. Zhang

    (State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China)

  • H.-Q. Lin

    (State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China)

  • C.-Y. Xu

    (State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China)

  • X.-C. Wang

    (State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China)

  • G.-Y. Wang

    (State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China)

  • J. Chen

    (State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China)

Abstract

Maize yield is severely affected by soil salinity. In an effort to engineer maize for improved salt tolerance, embryogenic calli of maize were co-bombarded with plasmids containing Oryza sativa Na+/H+ antiporter gene (OsNHX1) and bar genes. For the molecular analysis of putative transgenic samples, PCR, Southern and Northern blots were carried out. The maize plants over-expressing OsNHX1 accumulated more biomass when grown in the presence of 200mM NaCl in greenhouse conditions. Higher Na+ and K+ content was observed in transgenic leaves than in wildtype leaves when treated with 100~200mM NaCl, while the osmotic potential and the proline content in transgenic leaves was lower than in wild-type maize. A field trial revealed that the transgenic maize plants produced higher grain yields than the wild-type plants at the vegetative growth stage. These results demonstrate that the OsNHX1 gene was successfully transferred into Zea mays, and the salt-tolerance of transgenic maize was improved by overexpression of the OsNHX1 gene.

Suggested Citation

  • M. Chen & Q.-J. Chen & X.-G. Niu & R. Zhang & H.-Q. Lin & C.-Y. Xu & X.-C. Wang & G.-Y. Wang & J. Chen, 2007. "Expression of OsNHX1 gene in maize confers salt tolerance and promotes plant growth in the field," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 53(11), pages 490-498.
    • Handle: RePEc:caa:jnlpse:v:53:y:2007:i:11:id:2302-pse
    DOI: 10.17221/2302-PSE
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