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Microbial biomass-C determined using CaCl2 and K2SO4 as extraction reagents

Author

Listed:
  • L. Růžek

    (CzechUniversity of Agriculture in Prague, Czech Republic)

  • M. Nováková

    (CzechUniversity of Agriculture in Prague, Czech Republic)

  • K. Voříšek

    (CzechUniversity of Agriculture in Prague, Czech Republic)

  • I. Skořepová

    (CzechEcological Institute in Prague, Czech Republic)

  • L. Vortelová

    (CzechUniversity of Agriculture in Prague, Czech Republic)

  • Z. Kalfařová

    (CzechUniversity of Agriculture in Prague, Czech Republic)

  • J. Černý

    (CzechUniversity of Agriculture in Prague, Czech Republic)

  • T. Částka

    (CzechUniversity of Agriculture in Prague, Czech Republic)

  • W. Barabasz

    (University of Agriculture in Krakow, Poland)

Abstract

Microbial biomass-C [MBC] was determined by re-hydration [RHD] technique using two very similar salt solutions in dissociation potency (0.5 mol/l K2SO4 [MBC-K] and 0.01 mol/l CaCl2 [MBC-Ca]) in forest, grassland, arable Cambisols [Inceptisols] and Podzols [Spodosols]. MBC-Ca ranged from 254 to 5076 mg/kg dry soil (1.2-4.0% of Corg). 114 soil samples were examined in the years 2002 and 2003. Organic C compounds extracted by 0.5 mol/l K2SO4 [EC-K] and 0.01 mol/l CaCl2 [EC-Ca] increased in sequence: (1) arable Cambisols (100%), (2) cut and grazed grasslands (547%), (3) forest mineral horizon AH: 0-50 mm (783%) and (4) Norway spruce forest floor (2421%). The ratio EC-Ca/EC-K reached on average 62% and ranged from 48% to 74%. Correlation between EC-K and EC-Ca values is connected with soil organic matter status; the correlation was very close for Cambisols (r2 = 0.925), a medium correlation was found for forest floor (r2 = 0.380) and a weak correlation was observed for Podzols (r2 = 0.042). The correlation between MBC-K and MBC-Ca was very close in all cases: Cambisols (r2 = 0.811), Podzols (r2 = 0.904) and forest floor (r2 = 0.496). The ratio between organic carbon and organic nitrogen in 0.01 mol/l CaCl2 extracts [EC-Ca/Norg] could be declared as a new indicator for soil microbial association status.

Suggested Citation

  • L. Růžek & M. Nováková & K. Voříšek & I. Skořepová & L. Vortelová & Z. Kalfařová & J. Černý & T. Částka & W. Barabasz, 2005. "Microbial biomass-C determined using CaCl2 and K2SO4 as extraction reagents," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 51(10), pages 439-446.
    • Handle: RePEc:caa:jnlpse:v:51:y:2005:i:10:id:3615-pse
    DOI: 10.17221/3615-PSE
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    References listed on IDEAS

    as
    1. L. Růžek & K. Voříšek & S. Strnadová & M. Nováková & W. Barabasz, 2004. "Microbial characteristics, carbon and nitrogen content in cambisols and luvisols," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 50(5), pages 196-204.
    2. C. Aydinalp & A.V. Katkat, 2004. "The comparison of extraction methods for evaluating some heavy metals in polluted soils," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 50(5), pages 212-217.
    3. J. Hofman & J. Švihálek & I. Holoubek, 2004. "Evaluation of functional diversity of soil microbial communities - a case study," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 50(4), pages 141-148.
    4. S. Hejduk & F. Hrabě, 2003. "Influence of different systems of grazing, type of swards and fertilizing on underground phytomass of pastures," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 49(1), pages 18-23.
    5. J. Černý & J. Balík & D. Pavlíková & M. Zitková & K. Sýkora, 2003. "The influence of organic and mineral nitrogen fertilizers on microbial biomass nitrogen and extractable organic nitrogen in long-term experiments with maize," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 49(12), pages 560-564.
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    Cited by:

    1. M. Růžková & L. Růžek & K. Voříšek, 2008. "Soil biological activity of mulching and cut/harvested land set aside," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 54(5), pages 204-211.

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    More about this item

    Keywords

    microbial biomass; K2SO4 and CaCl2 extractable C; arable; forest; grassland soils; Cambisols; Inceptisols; Podzols; Spodosols; extraction methods;
    All these keywords.

    JEL classification:

    • C - Mathematical and Quantitative Methods

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