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The determination of coumestrol in alfalfa (Medicago sativa) by capillary electrophoresis

Author

Listed:
  • J. Moravcová

    (Institute of Chemical Technology, Prague, Czech Republic)

  • T. Kleinová

    (Institute of Chemical Technology, Prague, Czech Republic)

  • R. Loučka

    (Institute of Chemical Technology, Prague, Czech Republic)

Abstract

High performance capillary electrophoresis (HPCE) on an uncoated fused-silica capillary column using a borate buffer at pH 9.2 as electrolyte and diode-array detection was developed for the determination of coumestrol in alfalfa. The linear detector response was established in the concentration range 0.76-140 mg.dm-3, the minimum detectable limit was 0.39 mg.dm-3, and migration time of coumestrol was 5 min. 3-Isobutyl-1-methylxanthin was used as an internal standard. Coumestrol was isolated by acid extraction employing a mixture hydrochloric acid-acetonitrile at 95°C for 30 min followed by solid phase extraction. Relative standard deviations of reproducibility and repeatability were 1.77% and 5.49%, respectively. Spiking recovery value of 92% was achieved. Alfalfa, variety Morava, contains 148-248 mg.kg-1 coumestrol in dry matter. The proposed method is useful for routine analyses.

Suggested Citation

  • J. Moravcová & T. Kleinová & R. Loučka, 2002. "The determination of coumestrol in alfalfa (Medicago sativa) by capillary electrophoresis," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 48(5), pages 224-229.
    • Handle: RePEc:caa:jnlpse:v:48:y:2002:i:5:id:4230-pse
    DOI: 10.17221/4230-PSE
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