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Segregation of DNA markers of potato (Solanum tuberosum ssp. tuberosum L.) resistance against Ro1 pathotype Globodera rostochiensis in selected F1 progeny

Author

Listed:
  • S. Skupinová

    (Czech University of Agriculture in Prague, Czech Republic)

  • P. Vejl

    (Czech University of Agriculture in Prague, Czech Republic)

  • P. Sedlák

    (Czech University of Agriculture in Prague, Czech Republic)

  • J. Domkářová

    (Czech University of Agriculture in Prague, Czech Republic)

Abstract

Marker assisted selection is the fast and objective method for detection of resistance major genes. This method is practical for identification of some candidate genes of quantitative resistance. Genetic markers based on Polymerase Chain Reaction (PCR) were used for evaluation of F1 progenies Ornella × Mira and Tábor × Mira. Cultivar Mira has resistance against Ro1 pathotype G. rostochiensis. Cultivars Ornela and Tábor are susceptible to Ro1. Seedlings of F1 generations were used for analyses. Plants were cultivated in greenhouse. DNA was isolated from tissue discs by GenElute Plant Genomic DNA Kit (Sigma, SRN). PCR marker of major gene H1 was used for bulked analyses, according to (Niewöhner et al. 1995). Size of this marker was 760 bp. Standard infection tests with Ro1 pathotype G. rostochiensis according to Potoček (1987) in all of the analysed genotypes were made. Segregation ratios of F1 progenies were determined. These ratios have described segregation of resistance markers and segregation of traits in the biological test. The both methods of evaluation of potato's resistance were compared by correlation analyse. High correlations were found between occurrence of PCR marker for H1 and resistance to Ro1 in biological test. Coefficient of correlation r = 0.962 in F1 progeny Ornella × Mira and r = 0.964 in hybrids Tábor × Mira. Statistical evaluation of real ratios of segregation by infection tests and DNA markers with theoretical ratios of segregation in simplex and duplex H1 gen qualitative determined resistance was made as well. Resistant cultivar Mira as donor of simplex determined resistance was confirmed.

Suggested Citation

  • S. Skupinová & P. Vejl & P. Sedlák & J. Domkářová, 2002. "Segregation of DNA markers of potato (Solanum tuberosum ssp. tuberosum L.) resistance against Ro1 pathotype Globodera rostochiensis in selected F1 progeny," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 48(11), pages 480-485.
    • Handle: RePEc:caa:jnlpse:v:48:y:2002:i:11:id:4399-pse
    DOI: 10.17221/4399-PSE
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    Keywords

    potato; Solanum tuberosum ssp. tuberosum L.; DNA markers; PCR; Ro1 pathotype Globodera rostochiensis; resistance; major gene H1; bulked segregation analysis;
    All these keywords.

    JEL classification:

    • H1 - Public Economics - - Structure and Scope of Government

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