IDEAS home Printed from https://ideas.repec.org/a/caa/jnlpps/vpreprintid104-2024-pps.html

Biological activity of Paenibacillus polymyxa GT2 isolate from soil in Japan against anthracnose caused by Colletotrichum orbiculare in cucumber

Author

Listed:
  • Abdul Wali Haqyar

    (Laboratory of Plant Pathology, Faculty of Life and Environmental Science, Shimane University, Matsue, Japan)

  • Masatoshi Ino

    (The United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan)

  • Naoto Kimura

    (The United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan)

  • Takumi Okido

    (Department of Plant Protection, Faculty of Agriculture, Shaikh Zayed University, Khost, Afghanistan)

  • Junichi Kihara

    (Department of Plant Protection, Faculty of Agriculture, Shaikh Zayed University, Khost, Afghanistan)

  • Makoto Ueno

    (Department of Plant Protection, Faculty of Agriculture, Shaikh Zayed University, Khost, Afghanistan)

Abstract

Cucumber anthracnose is a destructive fungal disease caused by Colletotrichum orbiculare. Common control strategies include chemical fungicides. However, this can lead to the development of pathogenic resistance. Therefore, it is necessary to identify natural compounds or microorganisms to develop new chemicals and the biological control of fungal pathogens. Isolate GT2, a bacterial isolate from soil samples collected in Shimane Prefecture, Japan, significantly inhibited in vitro mycelial growth and conidial germination of C. orbiculare, indicating a fungicidal effect against this pathogen. Furthermore, anthracnose lesion formation was significantly suppressed without phytotoxicity when cucumber leaves were pretreated with a cell culture suspension of the isolate GT2 before inoculation with C. orbiculare. Bioautography of the culture filtrate (CF) of the isolate GT2 using thin-layer chromatography showed that the compound inhibiting C. orbiculare growth had an Rf value of 0.38. The effective compound in GT2-CF was ethyl acetate insoluble and heat-stable at 121 °C and has a molecular weight larger than 1 000 Da. In conclusion, Paenibacillus polymyxa GT2 demonstrated the potential for developing a new fungicide and biological agent against anthracnose disease caused by C. orbiculare.

Suggested Citation

  • Abdul Wali Haqyar & Masatoshi Ino & Naoto Kimura & Takumi Okido & Junichi Kihara & Makoto Ueno, . "Biological activity of Paenibacillus polymyxa GT2 isolate from soil in Japan against anthracnose caused by Colletotrichum orbiculare in cucumber," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 0.
    • Handle: RePEc:caa:jnlpps:v:preprint:id:104-2024-pps
    DOI: 10.17221/104/2024-PPS
    as

    Download full text from publisher

    File URL: http://pps.agriculturejournals.cz/doi/10.17221/104/2024-PPS.html
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/104/2024-PPS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to

    for a different version of it.

    More about this item

    Keywords

    ;
    ;
    ;
    ;

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlpps:v:preprint:id:104-2024-pps. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.