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Emergence of quarantine Tobacco ringspot virus in Impatiens walleriana in the Czech Republic

Author

Listed:
  • Jiban Kumar KUNDU

    (Division of Crop Protection and Plant Health, Crop Research Institute, Prague, Czech Republic)

  • Sebastien GADIOU

    (Division of Crop Protection and Plant Health, Crop Research Institute, Prague, Czech Republic)

  • Gabriela SCHLESINGEROVÁ

    (Division of Diagnostics, Central Institute for Supervising and Testing in Agriculture, Olomouc, Czech Republic)

  • Miroslava DZIAKOVÁ

    (Division of Diagnostics, Central Institute for Supervising and Testing in Agriculture, Olomouc, Czech Republic)

  • Václav ČERMÁK

    (Division of Diagnostics, Central Institute for Supervising and Testing in Agriculture, Olomouc, Czech Republic)

Abstract

Tobacco ringspot virus (TRSV) is a quarantine pathogen in Europe. During an official inspection in November 2011, Impatiens walleriana plants showing symptoms were found in a nursery in the Czech Republic. The causal agent of the disease was detected as Nepovirus group A by RT-PCR using specific primers of the Nepovirus group. Sequence analysis of PCR fragments confirms that the detected virus is TRSV. TRSV detection in these plants was further confirmed by ELISA and one-step RT-PCR using specific primers. The coat protein (CP) gene of the Czech TRSV isolate was sequenced, and the sequence analysis showed high identity of both nucleotide (99.28%) and amino acid (99.96%) levels compared with other known TRSV isolates from GenBank. Two amino acid motifs characteristic of nepoviruses, FDDY (FDAY) and FWGR (FYGR), were found equally at positions 80 and 497 of the TRSV CP genes, respectively, including the sequences described in this study.

Suggested Citation

  • Jiban Kumar KUNDU & Sebastien GADIOU & Gabriela SCHLESINGEROVÁ & Miroslava DZIAKOVÁ & Václav ČERMÁK, 2015. "Emergence of quarantine Tobacco ringspot virus in Impatiens walleriana in the Czech Republic," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 51(3), pages 115-123.
    • Handle: RePEc:caa:jnlpps:v:51:y:2015:i:3:id:3-2015-pps
    DOI: 10.17221/3/2015-PPS
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