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Blueberry red ringspot virus eliminated from highbush blueberry by shoot tip culture

Author

Listed:
  • Josef ŠPAK

    (Institute of Plant Molecular Biology, Biology Centre, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic)

  • Daniela PAVINGEROVÁ

    (Institute of Plant Molecular Biology, Biology Centre, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic)

  • Jaroslava PŘIBYLOVÁ

    (Institute of Plant Molecular Biology, Biology Centre, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic)

  • Vlastimila ŠPAKOVÁ

    (Institute of Plant Molecular Biology, Biology Centre, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic)

  • František PAPRŠTEIN

    (Research and Breeding Institute of Pomology Holovousy Ltd., Holovousy, Czech Republic)

  • Jiří SEDLÁK

    (Research and Breeding Institute of Pomology Holovousy Ltd., Holovousy, Czech Republic)

Abstract

In order to find an effective elimination method of Blueberry red ringspot virus (BRRV), shoot tips of 2-3 mm size originating from buds of highbush blueberry cv. Darrow naturally infected by BRRV and collected in winter months were regenerated and multiplied on woody-plant medium (WPM) with 1.5 mg/l zeatin and 20 g/l sucrose. Developed shoots tested negative by polymerase chain reaction (PCR) for BRRV were transferred onto a rooting medium consisting of WPM supplemented with 1 mg/l indole-3-butyric acid. Rooting plants were transferred into soil and tested twice by PCR 6 months apart. Ninety per cent eradication of BRRV was achieved while isolating shoot tips, multiplying them in one vegetative generation, and regenerating whole 50-60 cm high plants within 30 months. All plants developed rich fruits after their overwintering in a cold greenhouse and were tested BRRV negative in summer 2013.

Suggested Citation

  • Josef ŠPAK & Daniela PAVINGEROVÁ & Jaroslava PŘIBYLOVÁ & Vlastimila ŠPAKOVÁ & František PAPRŠTEIN & Jiří SEDLÁK, 2014. "Blueberry red ringspot virus eliminated from highbush blueberry by shoot tip culture," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 50(4), pages 174-178.
    • Handle: RePEc:caa:jnlpps:v:50:y:2014:i:4:id:84-2013-pps
    DOI: 10.17221/84/2013-PPS
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