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Biochemical characterisation of α-amylase in two aphid species, Aphis fabae Scopoli (Hemiptera: Aphididae) and A. gossypii Glover (Hemiptera: Aphididae)

Author

Listed:
  • Ali Darvishzadeh

    (Plant Protection Department, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran)

  • Ali Reza Bandani

    (Plant Protection Department, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran)

  • Seyed Qasem Mousavi

    (Plant Protection Department, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran)

Abstract

We identify and characterise α-amylases of the two aphid species, A. fabae and A. gossypi. To do this, α-amylases of the two insect species were extracted and their activities were determined using 1% soluble starch. Results showed that α-amylase, which hydrolyses starch, is present in both aphids. Also, it was shown that optimum pH and temperature for the α-amylases of both species is 7.0 and 40°C, respectively. Gel assays using zymogram analysis showed that in both aphid species more than one isoform (two isoforms) of α-amylases hydrolyszing carbohydrates are present.

Suggested Citation

  • Ali Darvishzadeh & Ali Reza Bandani & Seyed Qasem Mousavi, 2014. "Biochemical characterisation of α-amylase in two aphid species, Aphis fabae Scopoli (Hemiptera: Aphididae) and A. gossypii Glover (Hemiptera: Aphididae)," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 50(2), pages 84-89.
    • Handle: RePEc:caa:jnlpps:v:50:y:2014:i:2:id:71-2012-pps
    DOI: 10.17221/71/2012-PPS
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    Keywords

    aphids; gel assay; starch;
    All these keywords.

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