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Analysis of genetic diversity and phylogeny of partial coat protein domain in Czech and Italian GFLV isolates

Author

Listed:
  • Aleš EICHMEIER

    (Faculty of Horticulture in Lednice, Mendeleum - Institute of Genetics, Mendel University in Brno, Lednice, Czech Republic)

  • Miroslav BARÁNEK

    (Faculty of Horticulture in Lednice, Mendeleum - Institute of Genetics, Mendel University in Brno, Lednice, Czech Republic)

  • Miroslav PIDRA

    (Faculty of Horticulture in Lednice, Mendeleum - Institute of Genetics, Mendel University in Brno, Lednice, Czech Republic)

Abstract

The genetic diversity of Grapevine fanleaf virus (GFLV) was evaluated in 4 isolates sampled from naturally infected grapevines from South Moravia (Czech Republic) and 2 Italian isolates from Bari (Italy). Conserved regions within sequences in databases were found and new primers corresponding to these regions were designed and tested for RT-PCR amplification of the CP codifying region. After sequencing of obtained amplicons the similarity of isolates was analysed via alignments of sequences and by means of dendrograms

Suggested Citation

  • Aleš EICHMEIER & Miroslav BARÁNEK & Miroslav PIDRA, 2010. "Analysis of genetic diversity and phylogeny of partial coat protein domain in Czech and Italian GFLV isolates," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 46(4), pages 145-148.
    • Handle: RePEc:caa:jnlpps:v:46:y:2010:i:4:id:10-2010-pps
    DOI: 10.17221/10/2010-PPS
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