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Serine proteases in the extracellular preparations of Phytophthora infestans: does their presence relate to the aggressiveness of the pathogen?

Author

Listed:
  • J. Hamill

    (Department of Applied Plant Science, Queen's University, Belfast, BT9 5PX Northern Ireland, United Kingdom * Tel.: +44 28 9025 5254, Fax: +44 28 9066 8372, E-mail: j.a.hamill@qub.ac.uk)

  • C. Selby

    (Department of Applied Plant Science, Queen's University, Belfast, BT9 5PX Northern Ireland, United Kingdom * Tel.: +44 28 9025 5254, Fax: +44 28 9066 8372, E-mail: j.a.hamill@qub.ac.uk)

  • L.R. Cooke

    (Department of Applied Plant Science, Queen's University, Belfast, BT9 5PX Northern Ireland, United Kingdom * Tel.: +44 28 9025 5254, Fax: +44 28 9066 8372, E-mail: j.a.hamill@qub.ac.uk)

Abstract

In this study the aggressiveness of nine isolates of P. infestans was determined using detached leaflets from cultivars Bintje and Stirling. The growth of the isolates on the leaflets was recorded on a daily basis, for seven days, and an assessment of their aggressiveness could then be made. Extracellular preparations (ECPs) from the zoospore suspension of each isolate were used as a source of proteolytic activity. The ECPs were found to contain a level of serine protease activity using BTEE (N-Benzoyl-L-Tyrosine Ethyl Ester) as a substrate and recording the absorbance at 256 nm. The possible relationship between the serine protease activity and the aggressiveness of the isolate is discussed.

Suggested Citation

  • J. Hamill & C. Selby & L.R. Cooke, 2002. "Serine proteases in the extracellular preparations of Phytophthora infestans: does their presence relate to the aggressiveness of the pathogen?," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 38(SI1-6thCo), pages 102-103.
    • Handle: RePEc:caa:jnlpps:v:38:y:2002:i:si1-6thconfefpp:id:10329-pps
    DOI: 10.17221/10329-PPS
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