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Utilisation of immunochemical methods for detection of Colletotrichum spp. in strawberry

Author

Listed:
  • Jiřina Krátká

    (Research Institute of Crop Production - Division of Plant Medicine, Prague-Ruzyně, Czech Republic)

  • Blanka Pekárová-Kyněrová

    (Research Institute of Crop Production - Division of Plant Medicine, Prague-Ruzyně, Czech Republic)

  • Iva Kudlíková

    (Research Institute of Crop Production - Division of Plant Medicine, Prague-Ruzyně, Czech Republic)

  • Jakub Slováček

    (Research Institute of Crop Production - Division of Plant Medicine, Prague-Ruzyně, Czech Republic)

  • Michaela Zemánková

    (Research Institute of Crop Production - Division of Plant Medicine, Prague-Ruzyně, Czech Republic)

Abstract

Four polyclonal and two monoclonal antibodies were prepared and tested to detect a quarantine pathogen of strawberry - Colletotrichum acutatum. Only one of them, polyclonal IgG K91, was sensitive enough to recognize the pathogen. This antibody was genus-specific and did not cross-react with several other fungal pathogens of strawberry (Phytophthora fragariae, P. cactorum, Verticillium albo-atrum, Botrytis cinerea, Pythium ultimum). Four techniques, PTA-ELISA, dot blot, immunoprint and immunofluorescent microscopy were used to test the specifity and sensitivity of antibodies. After artificial infection of strawberry (cvs Elsanta, Vanda, and Kama), Colletotrichum acutatum was detected by PTA-ELISA, dot blot and immunoprint in roots, crowns, petioles and fruits in the latent stage of the disease. For reliable detection in the latent stage it is recommended to use at least two of the mentioned techniques.

Suggested Citation

  • Jiřina Krátká & Blanka Pekárová-Kyněrová & Iva Kudlíková & Jakub Slováček & Michaela Zemánková, 2002. "Utilisation of immunochemical methods for detection of Colletotrichum spp. in strawberry," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 38(2), pages 55-63.
    • Handle: RePEc:caa:jnlpps:v:38:y:2002:i:2:id:4850-pps
    DOI: 10.17221/4850-PPS
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