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Polyamines during somatic embryo development in Norway spruce (Picea abies [L.])

Author

Listed:
  • J. Malá

    (Forestry and Game Management Research Institute, Strnady, Czech Republic)

  • M. Cvikrová

    (Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic)

  • P. Máchová

    (Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic)

  • O. Martincová

    (Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic)

Abstract

Contents of free polyamines (putrescine, spermidine and spermine) were determined in different developmental stages of Norway spruce (Picea abies [L.] Karst.) somatic embryos by means of HPLC. Determinations were performed embryogenic tissue after 4 weeks of the growth on proliferation medium, after 2 and 5 weeks of the culturing on maturation medium, and 2 weeks after desiccation. Maturation of somatic embryos (after 5 weeks) was accompanied by increase of concentrations of putrescine (2.3 times) and spermidine (3.2 times). In comparison with above mentioned polyamines, spermine concentrations were significantly lower (4.3 times). Two weeks after desiccation, the concentrations of putrescine decreased 5.4 times and spermidine 2.2 times in comparison with mature embryos. To improve the efficiency of somatic embryogenesis of less responsive genotypes, the supplementation of growth media by polyamines is discussed.

Suggested Citation

  • J. Malá & M. Cvikrová & P. Máchová & O. Martincová, 2009. "Polyamines during somatic embryo development in Norway spruce (Picea abies [L.])," Journal of Forest Science, Czech Academy of Agricultural Sciences, vol. 55(2), pages 75-80.
    • Handle: RePEc:caa:jnljfs:v:55:y:2009:i:2:id:84-2008-jfs
    DOI: 10.17221/84/2008-JFS
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