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Content of trans-resveratrol, trans-ε-viniferin and trans-δ-viniferin in young spring grapevine canes - the influence of samples drying

Author

Listed:
  • Naděžda Vrchotová

    (Laboratory of Metabolomics and Isotopic Analyses, Global Change Research Institute, Brno, Czech Republic)

  • Jan Tříska

    (Laboratory of Metabolomics and Isotopic Analyses, Global Change Research Institute, Brno, Czech Republic)

  • Radomíra Střalková

    (Research Station for Viticulture Karlštejn, Crop Research Institute, Prague, Czech Republic)

  • Štěpán Horník

    (Department of Analytical Chemistry, Institute of Chemical Process Fundamentals, Prague, Czech Republic)

  • Jan Sýkora

    (Department of Analytical Chemistry, Institute of Chemical Process Fundamentals, Prague, Czech Republic)

  • Josef Balík

    (Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic)

  • Ivo Soural

    (Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic)

  • Lukáš Toupal

    (Department of Chemistry, Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic)

  • Radek Sotolář

    (Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic)

Abstract

The main objective was to study the effect of sample preparation on the content of trans-resveratrol, trans-ε-viniferin and trans-δ-viniferin in young spring grapevine canes. The samples of six varieties of Vitis vinifera L. ('Hibernal', 'Malverina', 'Kolor', 'Fioletovij Augustovskij', 'Grüner Veltliner' and 'Blaufränkisch') were either lyophilised or slow dried at room temperature (three months), extracted and analysed by HPLC using DAD and FLD detectors and by LC-MS. The presence of trans-δ-viniferin was confirmed by comparison with trans-δ-viniferin prepared by dimerization of trans-resveratrol using laccase and its structure was verified by LC-NMR. The slow drying of the samples at room temperature enables the synthesis of a significant amount of stilbenes in all the studied varieties of V. vinifera canes resulting in a higher stilbene content compared to the lyophilised samples. The results of this study show the importance of the method of drying the cane sample before the extraction and analysis.

Suggested Citation

  • Naděžda Vrchotová & Jan Tříska & Radomíra Střalková & Štěpán Horník & Jan Sýkora & Josef Balík & Ivo Soural & Lukáš Toupal & Radek Sotolář, 2022. "Content of trans-resveratrol, trans-ε-viniferin and trans-δ-viniferin in young spring grapevine canes - the influence of samples drying," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 49(3), pages 179-188.
    • Handle: RePEc:caa:jnlhor:v:49:y:2022:i:3:id:19-2021-hortsci
    DOI: 10.17221/19/2021-HORTSCI
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