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Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne

Author

Listed:
  • Monika Poniewozik

    (Institute of Horticultural Production, Faculty of Horticulture and Landscape Architecture, University of Life Sciences in Lublin, Lublin, Poland)

  • Marzena Parzymies
  • Paweł Szot

    (Institute of Horticultural Production, Faculty of Horticulture and Landscape Architecture, University of Life Sciences in Lublin, Lublin, Poland)

Abstract

Phenolic compounds limit micropropagation of many orchids in vitro. The aim of the study was to estimate the effect of activated charcoal (AC);1, 2 or 4 g/L) or ascorbic acid (AA; 10, 20 or 30 mg/L) added to the half strength MS medium on the growth and o-dihydroxyphenols content in Paphiopedilum insigne in vitro. A positive effect of AC on the shoot and root formation has been found. The highest multiplication rate (5.6 shoots/explant) and rooting frequency were obtained on medium containing 2 g/L of AC. However, AC reduced the leaf number as compared to the control. The lowest content of o-dihydroxyphenols was marked in Paphiopedilum insigne leaves when the shoots were grown on medium with 10 mg/L AA, followed by AC at 1 or 2 g/L.

Suggested Citation

  • Monika Poniewozik & Marzena Parzymies & Paweł Szot, 2022. "Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 49(1), pages 48-51.
    • Handle: RePEc:caa:jnlhor:v:49:y:2022:i:1:id:68-2020-hortsci
    DOI: 10.17221/68/2020-HORTSCI
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