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Resolving the browning during the establishment of the in vitro propogation of Prunus avium cv. 'Fuchen'

Author

Listed:
  • Yinkai Xi

    (Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, Kunming, P.R. China
    Chemistry Department, Guizhou University of Traditional Chinese Medicine, Guiyang, P.R. China)

  • Biao Zeng

    (Medicinal Plants Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, P.R. China)

  • Hengyu Huang

    (Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, Kunming, P.R. China
    Qiucheng Breeding Company Limited, Lijiang, P.R. China)

  • Ye Wang

    (Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, Kunming, P.R. China
    Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, P.R. China)

  • Pinhui Yang

    (Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, Kunming, P.R. China)

Abstract

Prunus avium cv. 'Fuchen' is a cultivated species with a high-profile economic benefit. Four media were used as a basal media equipped with four exogenous hormones to establish an intact regeneration system. Sodium thiosulfate (Na2S2O3) and active carbon (AC) improved the browning phenomenon. The results indicated that 1.0 g/L of AC and 30 g/L of Na2S2O3 were the best combination to inhibit the browning on the optimal basal medium - olive medium (OM) (followed by woody plant medium). The OM containing 1.5 mg/L of zeatin, 2.0 mg/L of α-naphtalenaecetic acid (NAA) and 0.5 mg/L of kinetin was beneficial for the germination and growth of the axillary buds. Green bud points were visible at the base of the swollen base after 25 days with a high proliferation coefficient (more than 7.50). The most optimal medium for rooting was 1/2 OM combined with 1.0 mg/L of NAA and 0.5 mg/L of indole-3-butyric-acid with a 100% rooting rate. The survival rate was up to 100% after 60 days with acclimatisation. Generally, a high-effective regeneration system was established, which provides a reference for keeping the excellent traits of the cultivar.

Suggested Citation

  • Yinkai Xi & Biao Zeng & Hengyu Huang & Ye Wang & Pinhui Yang, 2022. "Resolving the browning during the establishment of the in vitro propogation of Prunus avium cv. 'Fuchen'," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 49(1), pages 1-9.
    • Handle: RePEc:caa:jnlhor:v:49:y:2022:i:1:id:51-2020-hortsci
    DOI: 10.17221/51/2020-HORTSCI
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    References listed on IDEAS

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    1. Dj. V. Ružić & T. I. Vujović, 2008. "The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.)," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 35(1), pages 12-21.
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