Author
Listed:
- Valbona Sota
(Department of Biotechnology, Faculty of Natural Sciences, Tirana, Albania)
- Carla Benelli
(Institute of BioEconomy, National Research Council (CNR/IBE), Sesto Fiorentino (Florence), Italy)
- Brunilda Çuko
(Department of Biotechnology, Faculty of Natural Sciences, Tirana, Albania)
- Elektra Papakosta
(Center of Agricultural Technologies Transfer, Vlora, Albania)
- Claudio Depaoli
(Laboratory of Micropropagation Predaia (Trento), Italy)
- Maurizio Lambardi
(Institute of BioEconomy, National Research Council (CNR/IBE), Sesto Fiorentino (Florence), Italy)
- Efigjeni Kongjika
(Section of Natural and Technical Sciences, Albanian Academy of Sciences, Tirana, Albania)
Abstract
Malus sylvestris (L.) Mill., an economically-important fruit tree, is native to Albania and in many parts of Europe. It is cultivated as an ornamental tree, while its fruits are collected for food and a source of antioxidant substances. It is included in The IUCN Red List of Threatened Species. For these reasons, it is very important to optimise a micropropagation protocol, in order to obtain great numbers of clonal plantlets for ex situ conservation and production purposes. A liquid culture in a temporary immersion system (TIS) is a recently-proposed system for large-scale in vitro plant propagation. In this study, lateral buds of M. sylvestris were inoculated in MS medium with BAP (1 mg/L) and NAA (0.1 mg/L). In order to avoid oxidative stress, different antioxidants were previously tested with the culture in a gelled medium, and the combination of ascorbic acid and citric acid (both at 100 mg/L) was selected for the following culture in TIS. Stabilised explants were then cultivated in ElecTIS, an innovative TIS bioreactor, and in a semisolid medium, after which the two culture systems were evaluated. Overall, the ElecTIS showed to be more effective for all the tested parameters.
Suggested Citation
Valbona Sota & Carla Benelli & Brunilda Çuko & Elektra Papakosta & Claudio Depaoli & Maurizio Lambardi & Efigjeni Kongjika, 2021.
"Evaluation of ElecTIS bioreactor for the micropropagation of Malus sylvestris (L.) Mill., an important autochthonous species of Albania,"
Horticultural Science, Czech Academy of Agricultural Sciences, vol. 48(1), pages 12-21.
Handle:
RePEc:caa:jnlhor:v:48:y:2021:i:1:id:69-2020-hortsci
DOI: 10.17221/69/2020-HORTSCI
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