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Analysis of freezable water content by DSC for apple dormant bud cryopreservation

Author

Listed:
  • Alois Bilavčík
  • Jiří Zámečník

    (Physiology and Cryobiology of Plants Department, Crop Research Institute, Prague, Czech Republic)

  • Miloš Faltus

    (Physiology and Cryobiology of Plants Department, Crop Research Institute, Prague, Czech Republic)

  • Petra Jadrná

    (Physiology and Cryobiology of Plants Department, Crop Research Institute, Prague, Czech Republic)

Abstract

Dormant bud cryopreservation is a valuable tool for the germplasm of temperate trees preservation. Freezable water content, as an important cryopreservation characteristic, was analysed by DSC. Scions of modern apple tree varieties (Malus domestica) were collected during the winter season from orchards, freeze-dehydrated to 26-32% of water content at -5°C and cryopreserved by the two-step procedure. Samples were rehydrated in moist peat after 4 months of cryopreservation and grafted on rootstocks in a field. Regeneration by sprouting buds on rootstocks was evaluated. The varieties were divided into three groups according to their water content and percentage of crystallized water. The regeneration in the three groups decreased with decreasing bud water content. Regeneration was higher than 80% in most of the varieties and reached 100% in almost 1/3 of the tested varieties. In only one variety, was regeneration lower than 40%, which is still a high regeneration rate for considering apple genotypes as successfully cryopreserved. According to this study, the two-step cryoprotocol was successfully optimized for Central European conditions.

Suggested Citation

  • Alois Bilavčík & Jiří Zámečník & Miloš Faltus & Petra Jadrná, 2019. "Analysis of freezable water content by DSC for apple dormant bud cryopreservation," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 46(4), pages 163-170.
    • Handle: RePEc:caa:jnlhor:v:46:y:2019:i:4:id:146-2018-hortsci
    DOI: 10.17221/146/2018-HORTSCI
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    References listed on IDEAS

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    1. Dj. Ružić & T. Vujović & R. Cerović, 2014. "Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 41(2), pages 55-63.
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