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In vitro elimination of Black raspberry necrosis virus from black raspberry (Rubus occidentalis) - Short Communication

Author

Listed:
  • E.J. Cheong

    (National Germplasm Resources Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, USA)

  • A.R. Jeon

    (National Germplasm Resources Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, USA)

  • J.W. Kang

    (National Germplasm Resources Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, USA)

  • R. Mock

    (National Germplasm Resources Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, USA)

  • G. Kinard

    (National Germplasm Resources Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, USA)

  • R. Li

    (National Germplasm Resources Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, USA)

Abstract

Black raspberry necrosis virus (BRNV) is one of the most important viral pathogens of Rubus spp. In this study, a procedure combining in vitro culture and heat therapy on axillary buds of Rubusoccidentalis was developed to eliminate BRNV from infected plants. Axillary buds were grown aseptically at 4-h alternating periods of 29°C and 38°C with 14-h day length for 1-5 weeks. Shoots induced after thermotherapy were tested for the viruses by RT-PCR. BRNV was not detected in any shoots indicating that BRNV was heat labile. BRNV-free shoots were rooted in vitro and acclimatized in greenhouse. Regenerated plants remained virus-free after 2-month acclimatization, 3-month dormancy and 5-month growth periods.

Suggested Citation

  • E.J. Cheong & A.R. Jeon & J.W. Kang & R. Mock & G. Kinard & R. Li, 2014. "In vitro elimination of Black raspberry necrosis virus from black raspberry (Rubus occidentalis) - Short Communication," Horticultural Science, Czech Academy of Agricultural Sciences, vol. 41(2), pages 95-99.
    • Handle: RePEc:caa:jnlhor:v:41:y:2014:i:2:id:266-2013-hortsci
    DOI: 10.17221/266/2013-HORTSCI
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