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Analysis of cashmere goat meat by label-free proteomics shows that MYL3 is a potential molecular marker of meat toughness

Author

Listed:
  • Yuchun Xie

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
    Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Autonomous Region, Hohhot, P.R. China
    Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, P.R. China)

  • Nai Rile

    (School of Agriculture and Forestry, Hulunbuir University, Hulunbuir, P.R. China)

  • Xuewu Li

    (School of Health and Wellness, Panzhihua University, Panzhihua, P.R. China)

  • Haijun Li

    (College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, P.R. China)

  • Meng Zhao

    (Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, P.R. China)

  • Tianyu Che

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
    Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Autonomous Region, Hohhot, P.R. China
    Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, P.R. China)

  • Ting Cai

    (Inner Mongolia Autonomous Region Agriculture and Animal Husbandry Technology Extension Center, Hohhot, P.R. China)

  • Zhihong Liu

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
    Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Autonomous Region, Hohhot, P.R. China
    Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, P.R. China)

  • Jinquan Li

    (College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
    Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Autonomous Region, Hohhot, P.R. China
    Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, P.R. China)

Abstract

The Inner Mongolia cashmere goat is famous for its bright white cashmere fibre. However, little attention is given to the excellent characteristics of this breed's meat. We used label-free proteomics to analyse the total protein content in five different muscles, and 1 227 proteins were detected. Through sequential windowed acquisition of all theoretical fragment ions (SWATH), 16, 33, 49, 39, and 31 differentially expressed proteins were successfully detected in the five muscles. Protein-protein interaction network analysis of differentially expressed proteins revealed many strong interactions related to fatty acid beta oxidation and muscle development. Based on SWATH in five muscles, 25 differentially expressed proteins related to muscle development were detected, including seven muscle fibre structural proteins (ACTG2, ACTN4, TAGLN, MYL3, MYL1, MYL6B and MYH4). Finally, immunohistochemical analysis of MYL3 showed that the proportion of MYL3 may be a potential molecular marker for muscle toughness.

Suggested Citation

  • Yuchun Xie & Nai Rile & Xuewu Li & Haijun Li & Meng Zhao & Tianyu Che & Ting Cai & Zhihong Liu & Jinquan Li, 2022. "Analysis of cashmere goat meat by label-free proteomics shows that MYL3 is a potential molecular marker of meat toughness," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 67(4), pages 137-146.
    • Handle: RePEc:caa:jnlcjs:v:67:y:2022:i:4:id:61-2021-cjas
    DOI: 10.17221/61/2021-CJAS
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