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Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidation

Author

Listed:
  • Yotsapon Yangngam

    (Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand)

  • Suttiwat Chapanya

    (Lumphayaklang Livestock Semen Production Center, Lopburi, Thailand)

  • Thevin Vongpralub

    (Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand)

  • Wuttigrai Boonkum

    (Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand
    Network Center for Animal Breeding and Omics Research, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand)

  • Vibuntita Chankitisakul

    (Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand
    Network Center for Animal Breeding and Omics Research, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand)

Abstract

Silk sericin plays a protective role in a variety of mammalian cells during cryopreservation. This study aimed to determine the effects of sericin supplementation to a freezing extender on sperm quality and lipid peroxidation in dairy bull sperm cryopreservation. Each semen sample of five Holstein-Friesian crossbred bulls was divided into four aliquots and diluted in a tris-egg yolk extender supplemented with different concentrations of sericin [0%, 0.25%, 0.5%, and 1.0% (wt/vol)]. Sperm motility (CASA), viability, acrosome integrity, mitochondrial membrane potential (fluorescent staining) and lipid peroxidation (malondialdehyde - MDA test) were analysed. The results show that the 0.25% and 0.5% sericin groups had the highest total sperm motility (P < 0.05). Sperm viability, acrosome integrity, and mitochondrial function were higher in the group supplemented with 0.25% sericin compared to the control and 1.0% (P < 0.05). Sericin supplementation with 0.25% and 0.5% significantly decreased MDA concentrations compared with the control (P < 0.05). In conclusion, supplementation of the semen freezing extender with sericin at the concentration of 0.25% significantly improved the post-thaw semen quality and reduced lipid peroxidation in Holstein-Friesian crossbred bulls.

Suggested Citation

  • Yotsapon Yangngam & Suttiwat Chapanya & Thevin Vongpralub & Wuttigrai Boonkum & Vibuntita Chankitisakul, 2021. "Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidation," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 66(1), pages 13-20.
    • Handle: RePEc:caa:jnlcjs:v:66:y:2021:i:1:id:104-2020-cjas
    DOI: 10.17221/104/2020-CJAS
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