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Expression and localization of PIWI proteins in testis and ovary of domestic sheep

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  • Chunyan Li

    (Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. China
    College of Animal Science and Technology, Anhui Agricultural University, Hefei, P. R. China)

  • Qiuyue Liu

    (Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. China)

  • Xiangyu Wang

    (Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. China)

  • Wenping Hu

    (Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. China)

  • Deping Han

    (College of Veterinary Medicine, China Agricultural University, Beijing, P. R. China)

  • Joram Mwashigadi Mwacharo

    (Small Ruminant Genomics, International Centre for Agricultural Research in the Dry Areas (ICARDA), Addis Ababa, Ethiopia)

  • Caihong Wei

    (Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. China)

  • Mingxing Chu

    (Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. China)

  • Ran Di

    (Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. China)

Abstract

The piRNA-PIWI protein complex plays crucial roles in safeguarding the genome against inordinate transposon mobilization and regulation of embryonic development. A previous study indicated the presence of piRNA in sheep reproductive organs. However, the tissue distribution and cellular localization of PIWI proteins in sheep remains unclear. Therefore the present study aimed to explore the expression profiles of mRNAs of mammalian PIWI proteins (PIWIL1, PIWIL2, PIWIL4 and AGO3) in 9 tissues derived from adult male and female sheep. Results showed the expression of PIWIL1, PIWIL2, and PIWIL4 was significantly higher in the testis and ovary than in the other tissues. Immunohistochemistry analysis of testes indicated that each of the 4 proteins had specific cellular localizations, and some of the localizations were different from those of other species. All the proteins were mainly localized in the primary spermatocytes, suggesting that they are crucial for silencing of transposon to guarantee the integrity of the gamete genome during early stage of spermatogenesis. In the ovaries, the PIWI proteins were mainly localized in oocytes from antral follicles and leukocytes in ovarian blood. Our results provide insights to better understand the functions of PIWI proteins during spermatogenesis, oogenesis and immune defence in sheep.

Suggested Citation

  • Chunyan Li & Qiuyue Liu & Xiangyu Wang & Wenping Hu & Deping Han & Joram Mwashigadi Mwacharo & Caihong Wei & Mingxing Chu & Ran Di, 2020. "Expression and localization of PIWI proteins in testis and ovary of domestic sheep," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 65(3), pages 86-96.
    • Handle: RePEc:caa:jnlcjs:v:65:y:2020:i:3:id:7-2020-cjas
    DOI: 10.17221/7/2020-CJAS
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    1. Jane E. Freedman & Mark Gerstein & Eric Mick & Joel Rozowsky & Daniel Levy & Robert Kitchen & Saumya Das & Ravi Shah & Kirsty Danielson & Lea Beaulieu & Fabio C. P. Navarro & Yaoyu Wang & Timur R. Gal, 2016. "Diverse human extracellular RNAs are widely detected in human plasma," Nature Communications, Nature, vol. 7(1), pages 1-14, September.
    2. Jane E. Freedman & Mark Gerstein & Eric Mick & Joel Rozowsky & Daniel Levy & Robert Kitchen & Saumya Das & Ravi Shah & Kirsty Danielson & Lea Beaulieu & Fabio C. P. Navarro & Yaoyu Wang & Timur R. Gal, 2016. "Correction: Corrigendum: Diverse human extracellular RNAs are widely detected in human plasma," Nature Communications, Nature, vol. 7(1), pages 1-1, September.
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