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Comparison of the pituitary gland transcriptome in pregnant and non-pregnant goats (Capra hircus)

Author

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  • Qing Quan

    (College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
    College of Economy and Technology, Anhui Agricultural University, Hefei, P.R. China)

  • Lu Zhu

    (College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
    Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei, P.R. China)

  • Qi Zheng

    (College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
    Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei, P.R. China)

  • Hao Wu

    (College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
    Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei, P.R. China)

  • Jing Jing

    (College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
    Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei, P.R. China)

  • Qing Chen

    (College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
    Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei, P.R. China)

  • Ya Liu

    (College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
    Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei, P.R. China)

  • Fugui Fang

    (College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
    Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei, P.R. China)

  • Yunsheng Li

    (College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
    Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei, P.R. China)

  • Yunhai Zhang

    (College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
    Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei, P.R. China)

  • Yinghui Ling

    (College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
    School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, UK)

Abstract

Pregnancy is strictly regulated by neuronal and hormonal factors with an essential role being played by the pituitary gland. We screened for differentially expressed genes (DEGs) in the pituitary that function in goat gestational development. Pregnant (AWGp) and non-pregnant Anhui white goats (AWGn) were analysed by deep-sequencing technology. A total of 12 774 092 and 13 872 327 clear reads were obtained in the AWGp and AWGn libraries, respectively. A total of 2593 genes were labelled as significantly differentially expressed in AWGp compared to AWGn, including 2158 upregulated genes and 435 downregulated genes. These genes included follicle stimulating hormone beta (FSHB) and luteinizing hormone beta (LHB), which showed an involvement in reproductive regulation and downregulation (AWGp vs AWGn). Quantitative real-time PCR (qPCR) results validated the DEG data. Subsequent gene ontology analysis indicated that a large number of these DEGs function in cellular processes, cell structures, and cell binding. The DEGs were also found by Kyoto Gene and Genomic Encyclopaedia analysis to be significantly enriched in 54 pathways, including the GnRH and TGF-beta signalling pathways that affect cell proliferation and hormone secretion. These data also identify genes that may play a role in pregnancy and reproduction in the goat and thus provide avenues for future research.

Suggested Citation

  • Qing Quan & Lu Zhu & Qi Zheng & Hao Wu & Jing Jing & Qing Chen & Ya Liu & Fugui Fang & Yunsheng Li & Yunhai Zhang & Yinghui Ling, 2019. "Comparison of the pituitary gland transcriptome in pregnant and non-pregnant goats (Capra hircus)," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 64(10), pages 420-430.
    • Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:10:id:141-2019-cjas
    DOI: 10.17221/141/2019-CJAS
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    References listed on IDEAS

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    1. Nicholas Hatzirodos & Claire Glister & Katja Hummitzsch & Helen F Irving-Rodgers & Philip G Knight & Raymond J Rodgers, 2017. "Transcriptomal profiling of bovine ovarian granulosa and theca interna cells in primary culture in comparison with their in vivo counterparts," PLOS ONE, Public Library of Science, vol. 12(3), pages 1-17, March.
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    1. Lu Zhu & Jingtong Huang & Jing Jing & Qi Zheng & Qianyun Ji & Ya Liu & Jianping Ding & Fugui Fang & Yunsheng Li & Yunhai Zhang & Yinghui Ling, 2021. "Expression of microRNAs in the hypothalamus of pregnant and non-pregnant goats," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 66(5), pages 156-167.

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