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Expression of genes encoding zona pellucida glycoproteins and cortical granule distribution in porcine oocytes isolated from small and medium follicles in relation to puberty status of donors

Author

Listed:
  • Michal Jeseta

    (Department of Obstetrics and Gynecology, University Hospital and Masaryk University, Brno, Czech Republic)

  • Joanna Budna

    (Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland)

  • Wiesława Kranc

    (Department of Anatomy, Poznan University of Medical Sciences, Poznan, Poland)

  • Sarka Hanulakova

    (Veterinary Research Institute, Brno, Czech Republic)

  • Artur Bryja

    (Department of Anatomy, Poznan University of Medical Sciences, Poznan, Poland)

  • Adrian Chachuła

    (Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland)

  • Sylwia Ciesiółka

    (Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland)

  • Katarzyna Wojtanowicz-Markiewicz

    (Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland
    Institute of Veterinary Sciences, Poznan University of Life Sciences, Poznan, Poland)

  • Dorota Bukowska

    (Institute of Veterinary Sciences, Poznan University of Life Sciences, Poznan, Poland)

  • Klaus-Peter Brüssow

    (Institute of Veterinary Sciences, Poznan University of Life Sciences, Poznan, Poland)

  • Małgorzata Bruska

    (Department of Anatomy, Poznan University of Medical Sciences, Poznan, Poland)

  • Michał Nowicki

    (Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland)

  • Paweł Antosik

    (Institute of Veterinary Sciences, Poznan University of Life Sciences, Poznan, Poland)

  • Pavlina Hulinska

    (Veterinary Research Institute, Brno, Czech Republic)

  • Marie Machatkova

    (Veterinary Research Institute, Brno, Czech Republic)

  • Maciej Zabel

    (Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland
    Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland)

  • Bartosz Kempisty

    (Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland
    Department of Anatomy, Poznan University of Medical Sciences, Poznan, Poland)

Abstract

The zona pellucida proteins belong to a group of proteins that regulate the processes of gamete recognition, interaction, and fusion, since they are recognized as primary and secondary sperm receptors. It is suggested that cortical granule distribution is significantly associated with the zona pellucida structure and membrane preparation to cortical and acrosome reaction. Therefore, this study investigated the zona pellucida marker gene expression (ZP2 and ZP4 protein) in relation to cortical granules distribution in oocytes and puberty status of donors. Oocytes were collected from adult cyclic sows (isolated from medium and small follicles) and juvenile gilts (isolated only from small follicles). The oocytes were examined by RT-qPCR and by confocal microscopy. The expression of genes for ZP2 and ZP4 protein in oocytes collected from small follicles from cycling sows was higher in comparison to oocytes from medium follicles or oocytes collected from small follicles from juvenile gilts (P < 0.001). We also observed increased expression of both ZP2 and ZP4 mRNA in oocytes collected from small follicles (juvenile gilts) as compared to medium follicles (cycling sows) (P < 0.001). Moreover, we found a difference in the distribution of cortical granules. In oocytes from medium follicles, the peripheral localization of cortical granules was twice higher than their central concentration. However, in oocytes derived from small follicles (cyclic sows) cortical granules in comparison to oocytes from medium follicles were more centrally localized (P < 0.05). It has been suggested that the donor puberty status and the size of follicles significantly influenced the zona pellucida gene expression and cortical granule localization in porcine oocytes. This is accompanied by fertilization specificity and fertilizability of porcine oocytes in vitro.

Suggested Citation

  • Michal Jeseta & Joanna Budna & Wiesława Kranc & Sarka Hanulakova & Artur Bryja & Adrian Chachuła & Sylwia Ciesiółka & Katarzyna Wojtanowicz-Markiewicz & Dorota Bukowska & Klaus-Peter Brüssow & Małgorz, 2017. "Expression of genes encoding zona pellucida glycoproteins and cortical granule distribution in porcine oocytes isolated from small and medium follicles in relation to puberty status of donors," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 62(6), pages 234-241.
    • Handle: RePEc:caa:jnlcjs:v:62:y:2017:i:6:id:90-2016-cjas
    DOI: 10.17221/90/2016-CJAS
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    References listed on IDEAS

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    1. M. Machatkova & P. Hulinska & J. Horakova & Z. Reckova & K. Hanzalova, 2008. "Oestrous cycle stage influences the morphology and maturation of porcine oocytes in vitro," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 53(2), pages 70-76.
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