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A rapid and cost-effective procedure for delineation and utilization of genomic microsatellites for paralleled genotyping in Vicia faba

Author

Listed:
  • Jelena M. ALEKSIĆ

    (Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia)

  • Bojana BANOVIĆ

    (Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia)

  • Jovanka MILJUŠ-ĐUKIĆ

    (Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia)

  • Živko JOVANOVIĆ

    (Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia)

  • Aleksandar MIKIĆ

    (Institute of Field and Vegetable Crops (IFVCNS), Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia)

  • Branko ĆUPINA

    (Department of Field and Vegetable Crops, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia)

  • Bojan ZLATKOVIĆ

    (Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Niš, Serbia)

  • Snežana ANĐELKOVIĆ

    (Institute for Forage Crops (IFC), Kruševac, Serbia)

  • Ilaria SPANU

    (Institute of Biosciences and Bioresources (IBBR) UOS, Sesto Fiorentino, Florence, Italy)

  • Mihailo JELIĆ

    (Department of Genetics and Evolution, Faculty of Biology, University of Belgrade, Belgrade, Serbia)

  • Vesna MAKSIMOVIĆ

    (Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia)

Abstract

Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important food and feed grain crop legume, they have not yet been used for comprehensive molecular characterization of this crop. We report a three-step procedure for rapid and cost-effective delineation and utilization of informative genomic nuclear SSRs for paralleled genotyping in faba bean suitable also for other species: (i) pre-selection of loci generating PCR products of expected lengths which are potentially polymorphic (achieved by PCR amplification in bulked samples); (ii) exclusion of loci burdened with persistent null alleles and multilocus amplification products (based on PCR amplification of pre-selected loci in individual genotypes), and (iii) multiplexing. We demonstrate also that genomic SSRs are promising molecular tools for molecular characterization of faba bean required also for crop improvement.

Suggested Citation

  • Jelena M. ALEKSIĆ & Bojana BANOVIĆ & Jovanka MILJUŠ-ĐUKIĆ & Živko JOVANOVIĆ & Aleksandar MIKIĆ & Branko ĆUPINA & Bojan ZLATKOVIĆ & Snežana ANĐELKOVIĆ & Ilaria SPANU & Mihailo JELIĆ & Vesna MAKSIMOVIĆ, 2015. "A rapid and cost-effective procedure for delineation and utilization of genomic microsatellites for paralleled genotyping in Vicia faba," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 51(1), pages 36-39.
    • Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:1:id:153-2014-cjgpb
    DOI: 10.17221/153/2014-CJGPB
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