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Protein extract of tobacco expressing StoVe1 gene inhibits Verticillium dahliae proliferation

Author

Listed:
  • Shui-Ping LIU

    (College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China)

  • Yong-Bo HONG

    (College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China)

  • Zhen WU

    (College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China)

  • Yong-Shuo MA

    (College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China)

  • Deng-Wei JUE

    (College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China)

  • Chao XIE

    (College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China)

  • Yan-Ping ZHU

    (College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China)

  • Min CHEN

    (College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China)

  • Qing YANG

    (College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China)

Abstract

Verticillium dahliae is a principal pathogen causing verticillium wilt in Solanaceae crops. StoVe1 is a gene resisting to verticillium wilt isolated from Solanum torvum. In order to generate resistant tobacco plants, StoVe1 was inserted in the orientation behind CaMV 35S promoter of vector pGS and this construct was introduced into tobacco by Agrobacterium-mediated transformation. A total of 12 kanamycin-resistant plants were generated and 7 independent transgenic lines were identified by PCR analysis. Quantitative RT-PCR analysis showed that the levels of StoVe1 transcript in transgenic lines were up to 2-6 fold higher than in the control. Anti-fungal assay indicated that the protein extract of transgenic tobacco lines showed strong inhibition activity to V. dahliae, 2 fold or higher compared to control plants. This result reveals that StoVe1, as a V. dahliae resistance gene, has an application potential in plant breeding for verticillium wilt resistance.

Suggested Citation

  • Shui-Ping LIU & Yong-Bo HONG & Zhen WU & Yong-Shuo MA & Deng-Wei JUE & Chao XIE & Yan-Ping ZHU & Min CHEN & Qing YANG, 2013. "Protein extract of tobacco expressing StoVe1 gene inhibits Verticillium dahliae proliferation," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 49(2), pages 58-64.
    • Handle: RePEc:caa:jnlcjg:v:49:y:2013:i:2:id:165-2012-cjgpb
    DOI: 10.17221/165/2012-CJGPB
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    References listed on IDEAS

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    1. Masoud TOHIDFAR & Robabeh HOSSAINI & Nemat SHOKHANDAN BASHIR & Tabatabaei MEISAM, 2012. "Enhanced resistance to Verticillium dahliae in transgenic cotton expressing an endochitinase gene from Phaseolus vulgaris," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 48(1), pages 33-41.
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