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Sequence analysis of the mtDNA region correlated with Shaan 2A cytoplasmic male sterility in rapeseed (Brassica napus L.)

Author

Listed:
  • Lenka HAVLÍČKOVÁ

    (Biotechnological Centre, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic)

  • Vladislav ČURN

    (Biotechnological Centre, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic)

  • Eva JOZOVÁ

    (Biotechnological Centre, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic)

  • Vratislav KUČERA

    (Crop Research Institute, Praha-Ruzyně, Czech Republic)

  • Miroslava VYVADILOVÁ

    (Crop Research Institute, Praha-Ruzyně, Czech Republic)

  • Miroslav KLÍMA

    (Crop Research Institute, Praha-Ruzyně, Czech Republic)

Abstract

Until now in Europe has not been cultivated any hybrid cultivar of oilseed rape based on the Shaan 2A cytoplasmic male sterility (CMS), a widely used CMS system in China. The aim of Czech breeders now is to produce new, improved cultivars of rapeseed based on this CMS system. Sterile Shaan 2A CMS line (S; rf/rf), its corresponding maintainers (N; rf/rf) and fertility restorers (S; Rf/Rf) were analyzed on molecular level for the presence of functional CMS cytoplasm. Two new primer pairs covering CMS-associated gene (so called orf224-1) present in Shaan 2A CMS line were developed and selection capability of the developed primers was successfully evaluated. These primers can be used for early selection of plants with functional Shaan 2A CMS system in breeding programmes.

Suggested Citation

  • Lenka HAVLÍČKOVÁ & Vladislav ČURN & Eva JOZOVÁ & Vratislav KUČERA & Miroslava VYVADILOVÁ & Miroslav KLÍMA, 2012. "Sequence analysis of the mtDNA region correlated with Shaan 2A cytoplasmic male sterility in rapeseed (Brassica napus L.)," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 48(3), pages 139-142.
    • Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:3:id:144-2012-cjgpb
    DOI: 10.17221/144/2012-CJGPB
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