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Multiplex PCR assay to detect rust resistance genes Lr26 and Lr37 in wheat

Author

Listed:
  • Taťána SUMÍKOVÁ

    (Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Alena HANZALOVÁ

    (Crop Research Institute, Prague-Ruzyně, Czech Republic)

Abstract

Multiplex PCR was developed and optimized for simultaneous detection of wheat leaf rust resistance genes Lr26 and Lr37. The presence of the genes was analyzed in 21 winter wheat cultivars registered in the Czech Republic. Gene Lr37 was detected in four tested cultivars (Bakfis, Biscay, Nicol, Mulan), gene Lr26 occurred only in one cultivar (Etela) and three cultivars (Clarus, Orlando and Rapsodia) were found to carry both these genes. Data obtained by PCR markers were compared with results of greenhouse and field tests. Seedling reactions of cultivars possessing Lr26 to seven different leaf rust isolates conformed to the results obtained by the marker analysis, however, there were found some discrepancies in the detections of Lr37, which could be detected in greenhouse seedling tests only with difficulties.

Suggested Citation

  • Taťána SUMÍKOVÁ & Alena HANZALOVÁ, 2010. "Multiplex PCR assay to detect rust resistance genes Lr26 and Lr37 in wheat," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 46(2), pages 85-89.
    • Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:2:id:32-2010-cjgpb
    DOI: 10.17221/32/2010-CJGPB
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    References listed on IDEAS

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    1. Alena HANZALOVÁ & Taťána SUMÍKOVÁ & Pavel BARTOŠ, 2009. "Determination of leaf rust resistance genes Lr10, Lr26 and Lr37 by molecular markers in wheat cultivars registered in the Czech Republic," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 45(2), pages 79-84.
    2. Monika Ambrozková & Francoise Dedryver & Veronika Dumalasová & Alena Hanzalová & Pavel Bartoš, 2002. "Determination of the cluster of wheat rust resistance genes Yr17, Lr37, and Sr38 by a molecular marker," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 38(2), pages 41-45.
    3. P. Bartoš & J. Ovesná & A. Hanzalová & J. Chrpová & V. Dumalasová & M. Škorpík & V. Šíp, 2004. "Presence of a Translocation from Aegilops ventricosa in Wheat Cultivars Registered in the Czech Republic," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 40(2), pages 31-35.
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