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SNP Typing in Cereals: Comparison of SSCP and SNaPShot Markers Using the Barley Mlo Locus as a Model

Author

Listed:
  • J. Ovesná

    (Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic)

  • K. Poláková

    (Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic)

  • L. Kučera

    (Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic)

  • J. Rulcová

    (Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic)

Abstract

We compared the applicability of SSCP and SNaPShot markers in barley Mlo loci as a model of SNP detection in cereals. Whereas the development of SSCP markers required optimisation steps, the ddNTP primer extension (SNaPShot) procedure based on knowledge of target sequence provided expected results without any previous optimisations. We have shown, that SNPs can be easily scored using the ABI PRISM 310 Genetic Analyser.

Suggested Citation

  • J. Ovesná & K. Poláková & L. Kučera & J. Rulcová, 2003. "SNP Typing in Cereals: Comparison of SSCP and SNaPShot Markers Using the Barley Mlo Locus as a Model," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 39(4), pages 109-112.
    • Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:4:id:3727-cjgpb
    DOI: 10.17221/3727-CJGPB
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