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In vitro cytotoxic and genotoxic effects of donkey milk on lung cancer and normal cells lines

Author

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  • Cetin Akca

    (Department of Biology, Cell Culture and Genetic Toxicology Laboratory, Faculty of Arts and Sciences, Uludag University, Bursa, Turkey)

  • Ozgur Vatan

    (Department of Biology, Cell Culture and Genetic Toxicology Laboratory, Faculty of Arts and Sciences, Uludag University, Bursa, Turkey)

  • Dilek Yilmaz

    (Department of Biology, Cell Culture and Genetic Toxicology Laboratory, Faculty of Arts and Sciences, Uludag University, Bursa, Turkey)

  • Huzeyfe Huriyet

    (Department of Biology, Cell Culture and Genetic Toxicology Laboratory, Faculty of Arts and Sciences, Uludag University, Bursa, Turkey)

  • Nilüfer Cinkilic

    (Department of Biology, Cell Culture and Genetic Toxicology Laboratory, Faculty of Arts and Sciences, Uludag University, Bursa, Turkey)

  • Tolga Cavas

Abstract

In vitro cytotoxic and genotoxic effects of donkey milk on cancer (A549) and normal (BEAS-2B) lung cell lines were investigated. The XTT and WST-1 tests as well as clonogenic assays were used to evaluate cytotoxicity. The comet assay and micronucleus test were used as genotoxicity endpoints. Donkey milk showed lower cytotoxic effects against normal lung cell line BEAS-2B in comparison to the tumor cell line A549. Genotoxicity experiments revealed dose dependent increases in the frequencies of micronuclei and single stranded DNA breaks in A549 cells whereas no significant damage was observed in BEAS-2B cells. The results indicate that donkey milk has anti-proliferative and genotoxic effects on lung cancer cells at concentrations which are non-toxic to normal lung cells.

Suggested Citation

  • Cetin Akca & Ozgur Vatan & Dilek Yilmaz & Huzeyfe Huriyet & Nilüfer Cinkilic & Tolga Cavas, 2019. "In vitro cytotoxic and genotoxic effects of donkey milk on lung cancer and normal cells lines," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 37(1), pages 29-35.
    • Handle: RePEc:caa:jnlcjf:v:37:y:2019:i:1:id:221-2018-cjfs
    DOI: 10.17221/221/2018-CJFS
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