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Investigation of hepatitis A and E viruses in mussels collected from the Bosphorus, in Istanbul, Turkey - short communication

Author

Listed:
  • Huseyin YILMAZ

    (Department of Virology)

  • Utku CIZMECIGIL

    (Department of Virology)

  • Eda Altan TARAKCI

    (Department of Virology)

  • Ozge AYDIN

    (Department of Virology)

  • Aysun YILMAZ

    (Department of Virology)

  • Mehmet CALICIOGLU

    (Department of Food Hygiene and Technology, Veterinary Faculty, University of Firat, Elazig, Turkey)

  • Gurhan CIFTCIOGLU

    (Department of Food Hygiene and Technology, Veterinary Faculty, University of Istanbul, Istanbul, Turkey)

  • Ali AYDIN

    (Department of Food Hygiene and Technology, Veterinary Faculty, University of Istanbul, Istanbul, Turkey)

  • Kamil BOSTAN

    (Department of Food Hygiene and Technology, University of Aydin, Istanbul, Turkey)

  • Tansel SIRELI

    (Department of Food Hygiene and Technology, Veterinary Faculty, University of Ankara, Ankara, Turkey 6Biruni Laboratories, Centro lab., Istanbul, Turkey)

  • Mine GUZEL
  • Asiye KARAKULLUKCU

    (Department of Microbiology, Cerrahpasa Faculty of Medicine, University of Istanbul, Istanbul, Turkey)

  • Bekir KOCAZEYBEK

    (Department of Microbiology, Cerrahpasa Faculty of Medicine, University of Istanbul, Istanbul, Turkey)

  • Wim van der POEL

    (Central Veterinary Institute, Lelystad, The Netherlands)

  • Juergen RICHT

    (Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, USA)

  • Nuri TURAN

    (Department of Virology)

Abstract

Hepatitis A and E viruses (HAV and HEV) are recognised as food- and water-borne infections and shellfish are a well-documented source of these viruses. The presence of HAV and HEV in mussels has not previously been investigated in Bosphorus, Istanbul, Turkey, and therefore the aim of this study was to investigate the frequency of HAV and HEV in mussels collected from this location. A total of 92 pooled samples representing 736 mussels originating along the Bosphorus coast were collected from fish distributors. RNA was extracted using the RNeasy Plant Mini Kit and a TaqMan real-time RT-PCR was performed using primers specific to HAV and HEV. Amongst the 92 pooled samples tested, 3 (3.3%) were found to be positive for HAV by the TaqMan real-time RT-PCR. No HEV was detected in any of the mussels tested. A 174 bp product was observed on gel electrophoresis with HAV positive samples detected by SYBR Green real-time PCR after reverse transcription. This product was sequenced and confirmed to contain HAV sequences by alignment using the data in GenBank. This is the first report describing the presence of HAV in mussels harvested in the Bosphorus, Istanbul, Turkey, indicating the potential contamination of aquatic environment and risk to public health.

Suggested Citation

  • Huseyin YILMAZ & Utku CIZMECIGIL & Eda Altan TARAKCI & Ozge AYDIN & Aysun YILMAZ & Mehmet CALICIOGLU & Gurhan CIFTCIOGLU & Ali AYDIN & Kamil BOSTAN & Tansel SIRELI & Mine GUZEL & Asiye KARAKULLUKCU & , 2018. "Investigation of hepatitis A and E viruses in mussels collected from the Bosphorus, in Istanbul, Turkey - short communication," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 36(3), pages 215-220.
    • Handle: RePEc:caa:jnlcjf:v:36:y:2018:i:3:id:233-2017-cjfs
    DOI: 10.17221/233/2017-CJFS
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    References listed on IDEAS

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    1. Shenyang Gao & Dandan Li & Enhui Zha & Tiezhong Zhou & Shen Wang & Xiqing Yue, 2015. "Surveillance of Hepatitis E Virus Contamination in Shellfish in China," IJERPH, MDPI, vol. 12(2), pages 1-11, February.
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