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Effects of a bilberry preparation on selected cell lines of the digestive system

Author

Listed:
  • Kamila Borowiec

    (Department of Biotechnology, Human Nutrition and Science of Food Commodities, Faculty of Food Science and Biotechnology, University of Life Sciences, Lublin, Poland)

  • Dominik Szwajgier

    (Department of Biotechnology, Human Nutrition and Science of Food Commodities, Faculty of Food Science and Biotechnology, University of Life Sciences, Lublin, Poland)

  • Anna Olejnik

    (Department of Biotechnology and Food Microbiology, Faculty of Food Science and Nutrition Poznań University of Life Sciences, Poznań, Poland)

  • Katarzyna Kowalska

    (Department of Biotechnology and Food Microbiology, Faculty of Food Science and Nutrition Poznań University of Life Sciences, Poznań, Poland)

  • Zdzisław Targoński

    (Department of Biotechnology, Human Nutrition and Science of Food Commodities, Faculty of Food Science and Biotechnology, University of Life Sciences, Lublin, Poland)

Abstract

Bilberry is a valuable wild forest fruit harvested in many countries in Europe. The biological activities of bilberry include antioxidant, anticancer, antiviral, antibacterial, and anticholinesterase activities. This study examines the protective effects of a bilberry (BB) preparation on IEC-6, Caco-2, and HepG2 cell lines. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to study the cytotoxicity of BB. The genotoxicity was determined using single-cell microgel electrophoresis. The Ames test was employed to assay bilberry mutagenicity. No significant effects of BB (12.5-100 µg dry mass/ml) were observed on the viability of IEC-6, Caco-2, and HepG2 cells. There were no differences in the percentage of DNA in the comet tail between the cells treated with BB (100 µg dry mass/ml) and the control cells. However, a significant reduction of oxidative DNA damage in the HepG2 cells was found. BB exhibited neither mutagenic nor promutagenic effects. Our results suggest that bilberry can be a potential tool in the prevention of chronic diseases, without any undesired effects on the cells of the gastrointestinal tract.

Suggested Citation

  • Kamila Borowiec & Dominik Szwajgier & Anna Olejnik & Katarzyna Kowalska & Zdzisław Targoński, 2016. "Effects of a bilberry preparation on selected cell lines of the digestive system," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 34(4), pages 300-305.
    • Handle: RePEc:caa:jnlcjf:v:34:y:2016:i:4:id:375-2015-cjfs
    DOI: 10.17221/375/2015-CJFS
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