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Aqueous extraction of limonin from Citrus reticulate Blanco

Author

Listed:
  • Can Liu

    (Key Laboratory of the Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, P.R.China)

  • Jing Liu

    (Key Laboratory of the Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, P.R.China)

  • Yonghai Rong

    (Key Laboratory of the Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, P.R.China)

  • Nvyong Liang

    (Jiangmen Haolun Corporation, Jiangmen, P.R. China)

  • Long Rong

    (Key Laboratory of the Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, P.R.China)

Abstract

The replacement of organic solutions in the extraction of limonin from citrus seeds with an alkaline solution was investigated. This method was based on the reversible conversion of limonin to limonoate A-ring lactone via ring-opening of D-ring lactone at different pH values. The extraction conditions, optimised using Taguchi experimental design, were as follows: pH 11, temperature 70°C, alkaline solution/seeds ratio 20:1 (v/w), ultrasonic power 800 W for 30 minutes. A yield of 7.5 mg/g (limonin/citrus seeds) of 98% pure limonin was obtained.

Suggested Citation

  • Can Liu & Jing Liu & Yonghai Rong & Nvyong Liang & Long Rong, 2012. "Aqueous extraction of limonin from Citrus reticulate Blanco," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 30(4), pages 364-368.
  • Handle: RePEc:caa:jnlcjf:v:30:y:2012:i:4:id:108-2011-cjfs
    DOI: 10.17221/108/2011-CJFS
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