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Optimisation of solid-state fermentation of Aspergillus niger JL-15 for xylanase production and xylooligosaccharides preparation

Author

Listed:
  • Xian-Jun Dai

    (Department of Food Science, College of Life Science and)

  • Ming-Qi Liu

    (Department of Food Science, College of Life Science and)

  • Hong-Xiao Jin

    (College of Materials Science and Engineering, China Jiliang University, Hangzhou, China)

  • Ming-Yan Jing

    (Department of Animal Science, University of Manitoba, Winnipeg, Canada$2)

Abstract

The production of xylanase (XylA) by Aspergillus niger JL-15 in solid-state fermentation (SSF) on orange peel was optimised by the response surface methodology (RSM). The results revealed that four factors had significant effects on the XylA production (P < 0.05), that is the concentrations of the added glycerin and ammonium sulfate, the moisture content, and fermentation time. Exploying orange peel as the solid substrate, maximum xylanase activity (917.7 U/g dry fermentation product) was obtained at 4.2% glycerin, 3.1% (NH4)2SO4, 61% moisture content, and 73.4-h fermentation, this activity being close to the predicted one and 3.2 times higher than that of the basic medium (218.5 U/g). Optimum temperature and pH for XylA activity were 55°C and pH 5.0, respectively. SDS-PAGE analysis showed that the relative molecular mass of XylA was about 30.0 kDa. XylA exhibited Km and Vmax values of 9.24 mg/ml and 54.05 μmol/min/ml, respectively. XylA liberated mainly xylotriose from birchwood xylan and wheat bran, respectively. XylA was an endo-acting xylanase with transglycosylation activity, with the ability to hydrolyse, xylobiose, xylotriose, xylotetraose, xylopentaose, and xylohexaose.

Suggested Citation

  • Xian-Jun Dai & Ming-Qi Liu & Hong-Xiao Jin & Ming-Yan Jing, 2011. "Optimisation of solid-state fermentation of Aspergillus niger JL-15 for xylanase production and xylooligosaccharides preparation," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 29(5), pages 557-567.
  • Handle: RePEc:caa:jnlcjf:v:29:y:2011:i:5:id:103-2010-cjfs
    DOI: 10.17221/103/2010-CJFS
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