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Interferences of PCR effectivity: importance for quantitative analyse

Author

Listed:
  • Jan Hodek

    (Department of Molecular Biology, Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Jaroslava Ovesná

    (Department of Molecular Biology, Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Ladislav Kučera

    (Department of Molecular Biology, Crop Research Institute, Prague-Ruzyně, Czech Republic)

Abstract

Importance of the Polymerase chain reaction (PCR) have already crossed the border of mere target DNA sequence present or absence analysis. For number analyses e.g. Genetically Modified Organisms (GMOs) or gene expression assesment the DNA quantification is demanded. Real-time (or quantitative) PCR is the most used tool for nucleic acids quantification. PCR efficiency has relevant importance on DNA quantification - it should be almost same for each PCR and its value should varied between 90-100%. There are a lot of PCR enhancers and inhibitors well known. We described impact of used DNA solvent and used laboratory plastic on real-time PCR efficiency.

Suggested Citation

  • Jan Hodek & Jaroslava Ovesná & Ladislav Kučera, 2009. "Interferences of PCR effectivity: importance for quantitative analyse," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 27(SpecialIs), pages 42-49.
    • Handle: RePEc:caa:jnlcjf:v:27:y:2009:i:specialissue2:id:677-cjfs
    DOI: 10.17221/677-CJFS
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    Cited by:

    1. Aleš VRÁBLÍK & Jan HODEK & Josef SOUKUP & Kateřina DEMNEROVÁ & Jaroslava OVESNÁ, 2012. "Development and verification of PCR based assay to dectect and quantify garden pea lec gene," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 30(3), pages 247-257.

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