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Immunohistochemical detection of soya protein - optimisation and verification of the method

Author

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  • Matej POSPIECH

    (Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic)

  • Bohuslava TREMLOVÁ

    (Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic)

  • Eva RENČOVÁ

    (Veterinary Research Institute, Brno, Czech Republic)

  • Zdeňka RANDULOVÁ

    (Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic)

Abstract

A functional immunohistochemical method for soya proteins detection was developed. The procedure is based on the avidin-biotin complex (ABC) method that attains sufficient sensitivity. The method was verified by the analysis of the model samples of different forms of soya additives containing various concentrations of soya isolate. The detection limit of soya present in the model samples was 0.5%. Different possibilities of the background staining were tested. The best results were obtained with the background staining according to Calleja. The results were confirmed by the accredited indirect ELISA method. The method allows the identification of various forms of soya proteins such as isolates, texturates, concentrates, and flour.

Suggested Citation

  • Matej POSPIECH & Bohuslava TREMLOVÁ & Eva RENČOVÁ & Zdeňka RANDULOVÁ, 2009. "Immunohistochemical detection of soya protein - optimisation and verification of the method," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 27(1), pages 11-19.
    • Handle: RePEc:caa:jnlcjf:v:27:y:2009:i:1:id:2848-cjfs
    DOI: 10.17221/2848-CJFS
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    Citations

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    Cited by:

    1. Zuzana Řezáčová-Lukášková & Bohuslava Tremlová & Matej Pospiech & Eva Renčová & Zdeňka Randulová, 2010. "Immunohistochemical detection of wheat protein in model samples," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 28(6), pages 516-519.
    2. Javad Sadeghinezhad & Bahador Hajimohammadi & Farkhondeh Izadi & Fatemeh Yarmahmoudi & Rocco Latorre, 2015. "Evaluation of the morphologic method for the detection of animal and herbal content in minced meat," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 33(6), pages 564-569.
    3. Zdeňka Randulová & Bohuslava Tremlová & Zuzana Řezáčová-Lukášková & Matej Pospiech & Ivan Straka, 2011. "Determination of soya protein in model meat products using image analysis," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 29(4), pages 318-321.
    4. Matej Pospiech & Bohuslava Tremlová & Eva Renčová & Zdeňka Randulová & Zuzana Řezáčová Lukášková & Jana Pokorná, 2011. "Comparison of the results of the ELISA, histochemical, and immunohistochemical detection of soya proteins in meat products," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 29(5), pages 471-479.

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