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Optimisation of the PCR method for the detection of Campylobacter jejuni and Campylobacter coli in samples of ready-to-eat chicken meals

Author

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  • Zdeňka Šabatková

    (Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic
    Department of Biomagnetic Techniques, Institute of Systems Biology and Ecology, Academy of Science of the Czech Republic, České Budějovice, Czech Republic)

  • Kateřina Demnerová

    (Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic)

  • Jarmila Pazlarová

    (Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic)

Abstract

This work compared the use of polymerase chain reaction (PCR) and the conventional CSN/ISO/10272 culture-based methods in the detection of Campylobacter species in ready-to-eat meals made from chicken meat. PCR was carried out with the primers specific to C. jejuni, C. coli, C. lari, and was modified with an internal control. The detection of campylobacters by PCR was performed on both untreated and spiked samples of real food purchased in local stores. For PCR, the detection limit was 2 CFU/g after 48 h enrichment in Park and Sanders broth. Duplex PCR proved to be highly reliable in the detection of campylobacters in different food types. Without extra spiking, samples from a global fast food chain exhibited positive amplification of the PCR product while but negative results were obtained from the cultivation of the same samples.

Suggested Citation

  • Zdeňka Šabatková & Kateřina Demnerová & Jarmila Pazlarová, 2008. "Optimisation of the PCR method for the detection of Campylobacter jejuni and Campylobacter coli in samples of ready-to-eat chicken meals," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 26(4), pages 291-297.
    • Handle: RePEc:caa:jnlcjf:v:26:y:2008:i:4:id:8-2008-cjfs
    DOI: 10.17221/8/2008-CJFS
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