IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjf/v20y2002i2id3508-cjfs.html
   My bibliography  Save this article

Beta-galactosidase activity in psychrotrophic microorganisms and their potential use in food industry

Author

Listed:
  • P. Karasová

    (Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague,)

  • V. Spiwok

    (Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague,)

  • Š. Malá

    (Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague,)

  • B. Králová

    (Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague,)

  • N.J. Russell

    (Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague,)

Abstract

Twenty-one psychrotrophic resp. psychrophilic bacterial strains were screened for presence of b-galactosidase activity which showed 8 of them. b-Galactosidase activity of these strains was determined for 2 substrates - synthetic substrate (ONPG) and lactose - and also temperature profile of this enzyme was measured. b-Galactosidase from Arthrobacter sp. C2-2 not only proved the typical properties of cold-active enzyme, but it also preferred lactose as a substrate. Therefore, it was chosen for further isolation and purification and was found that it contains two b-galactosidase isoenzymes. One of them had strong preference for lactose and was able to catalyse transglycosylation reactions at low temperature. It has, thus, potential use in food technology.

Suggested Citation

  • P. Karasová & V. Spiwok & Š. Malá & B. Králová & N.J. Russell, 2002. "Beta-galactosidase activity in psychrotrophic microorganisms and their potential use in food industry," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 20(2), pages 43-47.
    • Handle: RePEc:caa:jnlcjf:v:20:y:2002:i:2:id:3508-cjfs
    DOI: 10.17221/3508-CJFS
    as

    Download full text from publisher

    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/3508-CJFS.html
    Download Restriction: free of charge
    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/3508-CJFS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/3508-CJFS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjf:v:20:y:2002:i:2:id:3508-cjfs. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.