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Lactococcus lactis subsp. lactis LTM 32, a new bacteriocin-producing strain isolated from Vietnamese fermented milk

Author

Listed:
  • T.M. DO

    (Institute of Chemical Technology - Department of Dairy and Fat Technology, Prague, Czech Republic)

  • M. Plocková

    (Institute of Chemical Technology - Department of Dairy and Fat Technology, Prague, Czech Republic)

  • J. Chumchalová

    (Institute of Chemical Technology - Department of Dairy and Fat Technology, Prague, Czech Republic)

Abstract

Screening for bacteriocin production of 60 strains of lactic acid bacteria (LAB) both isolated from various Vietnamese fermented food and obtained from the culture collection resulted in the detection of a bacteriocin-producing strain, Lactococcus lactis subsp. lactis LTM 32 isolated from Vietnamese fermented milk. This strain was identified and partly characterized. This bacteriocin inhibited not only closely relative strains of LAB but also strains of Listeria monocytogenes, Bacillus sp. including B. cereus, B. stearothermophilus, B. subtilis, B. pumilus and Staphylococcus aureus. It was heat stable at 121°C for 15 min, at 100°C for 120 min and stable during storage at -20°C and 4°C for 3 months. The bacteriocin was inactivated by some proteolytic enzymes, namely by proteinase K and pronase E in concentrations up to 0.5 mg/ml but not by α-amylase and pepsin. The optimal growth and bacteriocin production were achieved in M17L broth at 30°C and initial pH 6.8. The characteristics of bacteriocin-producing strain Lactococcus lactis subsp. lactis LTM 32 are of interest for food safety.

Suggested Citation

  • T.M. DO & M. Plocková & J. Chumchalová, 2001. "Lactococcus lactis subsp. lactis LTM 32, a new bacteriocin-producing strain isolated from Vietnamese fermented milk," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 19(5), pages 171-176.
    • Handle: RePEc:caa:jnlcjf:v:19:y:2001:i:5:id:6603-cjfs
    DOI: 10.17221/6603-CJFS
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    Cited by:

    1. Ivana SLOŽILOVÁ & Sabina PURKRTOVÁ & Michaela Kosová & Miroslava Mihulová & Eva ŠVIRÁKOVÁ & Kateřina DEMNEROVÁ, 2014. "Antilisterial activity of lactic acid bacteria against Listeria monocytogenes strains originating from different sources," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 32(2), pages 145-151.

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