In silico analysis of hypermethylation in CpGislands of UCHL1 gene’s promoter in nasopharyngeal carcinoma

Background and Objective: The methylation of Ubiquitin C-Terminal Hydrolase L1 (UCHL1) gene has been reported in many human cancers including nasopharyngeal carcinoma (NPC). In Vietnam, the methylation of UCHL1 gene’s promoter in NPC has not been demonstrated yet. In this study, a systematic literature revision was carried out to summarize the current evidences about the frequencies of UCHL-1 gene’s promoter methylation in NPC for further application in Vietnamese population. Methods: A systematic literature analysis was conducted based on the comprehensive studies. Moreover, many bioinformatic tools such as Methprimer, TFsearch, IDT OligoAnalyzer 3.1 were used to predict the CpG islands, transcriptional factors, and to pick up the MSP (Methylation-Specific PCR) primers. Results: Total of three previous studies were summarized and accessed for eligibility from literature research. As the results, the average weight methylated frequencies were 72.4% and 13.0% for NPC and non-cancerous samples, respectively. The significant association between UCHL-1 promoter methylation and NPC with the OR of 10.459 (95% CI = 4.915 – 22.254, p