IDEAS home Printed from https://ideas.repec.org/a/bjw/techen/v15y2025i2p76-84.html

Optimization of indole-3-acetic acid production by Bacillus subtilis strain IA3

Author

Listed:
  • Tuan Ngoc Nguyen

    (Ton Duc Thang University, Ho Chi Minh City, Vietnam)

  • Linh Huynh Yen Luu

    (Ton Duc Thang University, Ho Chi Minh City, Vietnam)

Abstract

Indole-3-Acetic Acid (IAA) is a crucial plant growth-promoting hormone produced as a secondary metabolite by various microorganisms. The present study aims to isolate and screen IAA-producing endospore-forming bacteria from soil samples collected in Vietnam. A total of ten bacteria were isolated, and they were shown to have the ability to produce IAA, with strain IA3 exhibiting the highest production. Strain IA3 was identified as Bacillus subtilis based on the 16S rRNA sequencing and subsequently used to optimize conditions for IAA production. To maximize IAA yield, key factors influencing production were analyzed using Box-Behnken design. The optimized conditions led to a maximum IAA production of 47.67 ± 0.78 g/mL under the following conditions: 15.65 g/L molasses, 1.06 g/L (NH4)2SO4, and 0.52 g/L tryptophan. Therefore, Bacillus subtilis IA3 has potential applications for stimulating and enhancing plant growth or crop production.

Suggested Citation

  • Tuan Ngoc Nguyen & Linh Huynh Yen Luu, 2025. "Optimization of indole-3-acetic acid production by Bacillus subtilis strain IA3," HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY, HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE, HO CHI MINH CITY OPEN UNIVERSITY, vol. 15(2), pages 76-84.
    • Handle: RePEc:bjw:techen:v:15:y:2025:i:2:p:76-84
    DOI: 10.46223/HCMCOUJS.tech.en.15.2.4232.2025
    as

    Download full text from publisher

    File URL: https://journalofscience.ou.edu.vn/index.php/tech-en/article/view/4232/2424
    Download Restriction: no
    File URL: https://libkey.io/10.46223/HCMCOUJS.tech.en.15.2.4232.2025?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Keywords

    ;
    ;
    ;

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:bjw:techen:v:15:y:2025:i:2:p:76-84. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Vu Tuan Truong (email available below). General contact details of provider: https://journalofscience.ou.edu.vn/index.php/tech-en .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.