Antibacterial Effects of Tulsi (Ocimum Sanctum) on Isolated Resistant Bacteria Staphylococcus Aureus from Infected Cow

The current research work was carried out to screen and evaluate antimicrobial activity of leaf extracts of Ocimum sanctum (common name: Tulsi) on Multidrug resistant Staphylococcus aureus organism. Tulsi materials which were used during this study were collected from local garden whereas the bacterial strains were isolated from infected cow. Fresh leaves were processed and extracted by absolute ethanol and methanol before testing. Besides, antibiogram study performed and Minimum Inhibitory Concentration (MIC) was determined by diffusion method. Disk diffusion methods were used to determine zone diameter of inhibition (ZDI). ‘Four S. aureus presented resistance to these antibiotics: oxytetracycline, penicillin, streptomycin, amoxicillin, and erythromycin. These isolates were susceptible to tulsi extracted pellet’. The results showed that the effectiveness of the extracts was dependent of the concentration used thus the increase of extract concentration increased the inhibition zone against multidrug-resistant S. aureus (MDRSA) as antimicrobial agents against MDRSA and can be used to explore novel antibacterial compounds against MDRSA. However, Maximum ZDI (17mm) value was observed for methanol extracts 0.8g/ml against MDRSA and minimum ZDI (7mm) value was observed for methanol extracts 0.4g/ml against MDRSA and no zone was found 0.2g/ml of methanol etract of tulsi. From the current research, it can be concluded that the selected O. sanctum have great potential as antimicrobial agents against MDRSA Staphylococcus aureus and can be used to explore novel antibacterial compounds against MDRSA.