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Evaluation of the Gelling Property of Some Local Plants Seeds as Alternative Gelling Agents to Agar-Based Microbial Culture Media

Author

Listed:
  • Udofa, Edidiong Jumbo

    (Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, University of Uyo, Akwa Ibom State, Nigeria.)

  • Thomas, Paul Sunday

    (Department of Pharmacognosy and Natural Medicine, Faculty of Pharmacy, University of Uyo, Akwa Ibom State, Nigeria.)

  • Alozie, Mfonobong Favour

    (Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, University of Uyo, Akwa Ibom State, Nigeria.)

  • Nkanang, Abigail Johnny

    (Department of Microbiology, Faculty of Sciences, University of Uyo, Akwa Ibom State, Nigeria..)

  • Ekong, Ubong Samuel

    (Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, University of Uyo, Akwa Ibom State, Nigeria)

Abstract

Agar, an expensive gelling agent for culturing microbes affects holistic microbiological and pharmaceutical usage. Hence, plants used as thickening agents should be investigated. This work aims to evaluate the gelling properties of the seeds of Brachystegia eurycoma, Mucuna sloanei, Detarium microcarpum and Cucumeropsis mannii, as alternative gelling agents to agar in microbial culture media. The analyses were investigated in accordance with standard procedures and their modifications by pulverization, bleaching and physicochemical methods. The 3 plants showed relative gelling property when compared to that of agar-based media except for Cucumeropsis mannii which did not exhibit any gelling property at all concentrations. At the least (0.056g/mL) and highest (0.081g/mL) concentrations with equivalent gelling activity to agar at 0.015g/mL as the standard, B. eurycoma exhibited significant (p

Suggested Citation

  • Udofa, Edidiong Jumbo & Thomas, Paul Sunday & Alozie, Mfonobong Favour & Nkanang, Abigail Johnny & Ekong, Ubong Samuel, 2024. "Evaluation of the Gelling Property of Some Local Plants Seeds as Alternative Gelling Agents to Agar-Based Microbial Culture Media," International Journal of Research and Innovation in Applied Science, International Journal of Research and Innovation in Applied Science (IJRIAS), vol. 9(12), pages 312-325, December.
    • Handle: RePEc:bjf:journl:v:9:y:2024:i:12:p:312-325
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