Analytical Method Development and Validation of Nelumbo Nucifera Ethanolic Seed Powder Extract by UV-Visible Spectrophotometry

A precise, accurate, sensitive, and simple UV-Spectrophotometric method was established and validated for the estimation of Nelumbo Nucifera ethanolic seed powder extract. Ethanol was employed as a solvent in Soxhlet extraction process. The ethanolic extract of Nelumbo nucifera seed Powder has been extensively worked out for their therapeutic value with antioxidant and anti-inflammatory activity. UV spectroscopyisa quick and effective technique for the phytochemical analysis and confirmation of the occurrence of bioactive constituents in such extracts. Method development and validation require optimization of parameters such as wavelength selection, solvent compatibility, and calibration curves to obtain accurate measurement of active constituents. Standard stock solutions and working standard solutions were prepared according to the given procedure and Ethanol was employed as a solvent to prepare the working standard solutions of the range of 10-60 µg/ml and the λ max was 215nm. The range was found to be 10-60µg/ml and the correlation coefficient was 0.01x+0.0288. The equation for regression was calculated as 0.9934. The recovery came out to be between the range of 100.6%. The sensitivity of the method was confirmed by the method of performing LOD and LOQ values 1.16 µg/ml &3.50 µg/ml respectively for the Nelumbo Nucifera Drug.