A Review on Analytical Method Development and Validation for Simultaneous Estimation of Perindopril and Indapamide in Bulk and Pharmaceutical Dosage Form by RP-HPLC

Perindopril and Indapamide, a fixed-dose combination medication, are utilized for the management of hypertension by integrating the properties of an angiotensin-converting enzyme (ACE) inhibitor with a thiazide-like diuretic. The detection of Indapamide, Perindopril, and its active metabolite perindoprilat in human plasma or whole blood was achieved through hyphenated ultra-performance liquid chromatography-mass spectrometry (UPLC-MS/MS). Internal standards included indapamide-d3, perindopril-d4, and perindoprilat-d4. The chromatography was conducted at a column temperature of 50°C with a flow rate of 0.6 mL/min using isocratic elution, and UV detection was performed at 215 nm. The method demonstrated acceptable linearity, accuracy, and precision across concentration ranges of 1-5μg/mL for Perindopril and 10-15μg/mL for Indapamide. A statistical comparison of the proposed chromatographic method against reference methods was conducted using one-way analysis of variance, with Beer-Lambert’s range established at 0-60 μg/mL.